A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice.
A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice.
复制标题
一种定量PCR方法，可检测转基因小鼠中与肿瘤发生相关的血液microRNA。
DOI：
10.1186/1476-4598-7-74
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发表时间：
2008-09-30
期刊：
影响因子：
37.3
通讯作者：
Felsher, Dean W.
中科院分区：
文献类型：
--
作者：
Fan, Alice C.;Goldrick, Marianna M.;Ho, Jennifer;Liang, Yu;Bachireddy, Pavan;Felsher, Dean W.
MicroRNA (miRNA) 失调经常发生在癌症中。肿瘤模型中全血 miRNA 的分析尚未得到广泛报道，但可能会带来癌症早期检测和监测的新方法。为了确定是否可以在外周血中检测到与恶性肿瘤相关的 miRNA，我们使用实时逆转录酶 PCR 来确定从患有 c-MYC 诱导的淋巴瘤、肝细胞癌和骨肉瘤的转基因小鼠获得的全血中的 miRNA 谱。我们的研究中使用的基于 PCR 的检测仅需要 10 纳克的总 RNA，从而可以随着时间的推移对个体动物进行连续的微型分析（20 – 30 个 miRNA）。测量了处于 MYC 诱导的淋巴瘤发生和消退不同阶段的小鼠的血液 miRNA。无监督的数据分层聚类确定了与肿瘤类型和阶段相关的特定 miRNA 表达谱。发现患有肿瘤的小鼠血液中发生改变的 miRNA 在肿瘤消退后经常恢复到正常水平。我们的结果表明，在肿瘤发生和肿瘤消退过程中可以检测到血液 miRNA 的特定变化。
MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in the peripheral blood, we used real-time reverse transcriptase-PCR to determine miRNA profiles in whole blood obtained from transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma and osteosarcoma. The PCR-based assays used in our studies require only 10 nanograms of total RNA, allowing serial mini-profiles (20 – 30 miRNAs) to be carried out on individual animals over time. Blood miRNAs were measured from mice at different stages of MYC-induced lymphomagenesis and regression. Unsupervised hierarchical clustering of the data identified specific miRNA expression profiles that correlated with tumor type and stage. The miRNAs found to be altered in the blood of mice with tumors frequently reverted to normal levels upon tumor regression. Our results suggest that specific changes in blood miRNA can be detected during tumorigenesis and tumor regression.
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