Global profiling of ribosomal protein acetylation reveals essentiality of acetylation homeostasis in maintaining ribosome assembly and function.
Global profiling of ribosomal protein acetylation reveals essentiality of acetylation homeostasis in maintaining ribosome assembly and function.
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DOI：
10.1093/nar/gkad768
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发表时间：
2023-10-27
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
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作者：
乙酰化是一种全局性的翻译后修饰，调节各种细胞过程。细菌乙酰组学研究揭示了核糖体蛋白的广泛乙酰化。然而，乙酰化在调节核糖体功能中的作用仍然知之甚少。在这项研究中，我们系统地分析了核糖体蛋白乙酰化，并确定了52个核糖体蛋白（R-蛋白）从鼠伤寒沙门氏菌共289个乙酰化赖氨酸残基。大多数的乙酰化赖氨酸残基的r-蛋白被发现是由乙酰转移酶Pat和代谢中间体乙酰磷酸调节。我们的研究结果表明，乙酰化起着至关重要的作用，在装配成熟的70 S核糖体复合物通过调节r-蛋白结合rRNA。此外，适当的乙酰化对于延伸因子与多聚核糖体之间的相互作用以及调节核糖体翻译效率和保真度是重要的。乙酰化的失调可以改变细菌对核糖体靶向抗生素的敏感性。总的来说，我们的数据表明，核糖体的乙酰化稳态对其组装和功能至关重要。此外，这种机制可能代表了不同细胞类型对环境信号的普遍反应。
Acetylation is a global post-translational modification that regulates various cellular processes. Bacterial acetylomic studies have revealed extensive acetylation of ribosomal proteins. However, the role of acetylation in regulating ribosome function remains poorly understood. In this study, we systematically profiled ribosomal protein acetylation and identified a total of 289 acetylated lysine residues in 52 ribosomal proteins (r-proteins) from Salmonella Typhimurium. The majority of acetylated lysine residues of r-proteins were found to be regulated by both acetyltransferase Pat and metabolic intermediate acetyl phosphate. Our results show that acetylation plays a critical role in the assembly of the mature 70S ribosome complex by modulating r-proteins binding to rRNA. Moreover, appropriate acetylation is important for the interactions between elongation factors and polysomes, as well as regulating ribosome translation efficiency and fidelity. Dysregulation of acetylation could alter bacterial sensitivity to ribosome-targeting antibiotics. Collectively, our data suggest that the acetylation homeostasis of ribosomes is crucial for their assembly and function. Furthermore, this mechanism may represent a universal response to environmental signals across different cell types.
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影响因子：
16.6
作者：
Caban, Kelvin;Pavlov, Michael;Gonzalez, Ruben L., Jr.
通讯作者：
Gonzalez, Ruben L., Jr.
DOI：
10.1073/pnas.1525206113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Bullwinkle, Tammy J.;Ibba, Michael
通讯作者：
Ibba, Michael
影响因子：
4.4
作者：
Canals R;Xia XQ;Fronick C;Clifton SW;Ahmer BM;Andrews-Polymenis HL;Porwollik S;McClelland M
通讯作者：
McClelland M
影响因子：
64.5
作者：
Davis, Joseph H.;Tan, Yong Zi;Carragher, Bridget;Potter, Clinton S.;Lyumkis, Dmitry;Williamson, James R.
通讯作者：
Williamson, James R.
影响因子：
13.8
作者：
Guan, Kun-Liang;Xiong, Yue
通讯作者：
Xiong, Yue