Transcriptome analysis of Phelipanche aegyptiaca seed germination mechanisms stimulated by fluridone, TIS108, and GR24.
Transcriptome analysis of Phelipanche aegyptiaca seed germination mechanisms stimulated by fluridone, TIS108, and GR24.
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氟啶酮、TIS108 和 GR24 刺激埃及菲利普种子萌发机制的转录组分析
DOI：
10.1371/journal.pone.0187539
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhao SF
中科院分区：
文献类型：
--
作者：
Bao YZ;Yao ZQ;Cao XL;Peng JF;Xu Y;Chen MX;Zhao SF
埃及白杨是世界范围内最具破坏性的根部寄生植物之一，对许多作物物种造成严重损害。在自然条件下，埃及 P. aegyptiaca 种子必须经过调理，然后在发芽前受到宿主根部分泌物的刺激。然而，初步实验表明，TIS108（独脚金内酯的三唑型抑制剂）和氟啶酮（FL，类胡萝卜素生物合成抑制剂）均可以刺激埃及 P. aegyptiaca 种子的发芽，而无需水预处理步骤（即未处理的种子）。本研究的目的是利用深度 RNA 测序来了解更多关于 TIS108 和 FL 刺激未条件埃及 P. aegyptiaca 种子发芽的机制。进行深度 RNA 测序以比较以下处理中的发芽机制：(i) 未经过其他处理的未条件化埃及 P. aegyptiaca 种子，(ii) 用 100 mg/L TIS108 处理的未条件化种子，(iii) 用 100 mg/L FL + 100 mg/L GA3 处理的未条件化种子，(iv) 用无菌水处理的条件化种子，以及 (v) 用 0.03 mg/L 处理的条件化种子。 GR24。从头组装的转录组用于分析种子萌发过程中的转录动态。还确定了与发芽相关的关键基因类别。结果显示，与 TIS108 处理相比，条件处理中仅鉴定出 119 个差异表达基因。这表明在调理阶段满足了绝大多数发芽条件。脱落酸（ABA）和赤霉酸（GA）在埃及白杨发芽过程中发挥着重要作用。 TIS108、FL+GA3 和 GR24 刺激埃及 P. aegyptiaca 发芽的共同途径是同时降低 ABA 浓度和增加 GA 浓度。这些结果可能有助于鉴定更多能够刺激埃及 P. aegyptiaca 发芽的化合物。我们的转录组数据中还鉴定了 TIS108 的一些潜在靶位点。本实验结果表明TIS108和FL+GA3可用于通过自杀性萌发来控制埃及假单胞菌。
P. aegyptiaca is one of the most destructive root parasitic plants worldwide, causing serious damage to many crop species. Under natural conditions P. aegyptiaca seeds must be conditioned and then stimulated by host root exudates before germinating. However, preliminary experiments indicated that TIS108 (a triazole-type inhibitor of strigolactone) and fluridone (FL, an inhibitor of carotenoid-biosynthesis) both stimulated the germination of P. aegyptiaca seeds without a water preconditioning step (i.e. unconditioned seeds). The objective of this study was to use deep RNA sequencing to learn more about the mechanisms by which TIS108 and FL stimulate the germination of unconditioned P. aegyptiaca seeds. Deep RNA sequencing was performed to compare the mechanisms of germination in the following treatments: (i) unconditioned P. aegyptiaca seeds with no other treatment, (ii) unconditioned seeds treated with 100 mg/L TIS108, (iii) unconditioned seeds treated with 100 mg/L FL + 100 mg/L GA3, (iv) conditioned seeds treated with sterile water, and (v) conditioned seeds treated with 0.03 mg/L GR24. The de novo assembled transcriptome was used to analyze transcriptional dynamics during seed germination. The key gene categories involved in germination were also identified. The results showed that only 119 differentially expressed genes were identified in the conditioned treatment vs TIS108 treatment. This indicated that the vast majority of conditions for germination were met during the conditioning stage. Abscisic acid (ABA) and gibberellic acid (GA) played important roles during P. aegyptiaca germination. The common pathway of TIS108, FL+GA3, and GR24 in stimulating P. aegyptiaca germination was the simultaneous reduction in ABA concentrations and increase GA concentrations. These results could potentially aid the identification of more compounds that are capable of stimulating P. aegyptiaca germination. Some potential target sites of TIS108 were also identified in our transcriptome data. The results of this experiment suggest that TIS108 and FL+GA3 could be used to control P. aegyptiaca through suicidal germination.
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影响因子：
4.3
作者：
FONG, F;SCHIFF, JA
通讯作者：
SCHIFF, JA
影响因子：
4.2
作者：
Joel, Daniel M.;Bar, Hilla;Welbaum, Gregory E.
通讯作者：
Welbaum, Gregory E.
影响因子：
2.1
作者：
Malik, Heetika;Rutjes, Floris P. J. T.;Zwanenburg, Binne
通讯作者：
Zwanenburg, Binne
影响因子：
2.5
作者：
BARTELS, PG;WATSON, CW
通讯作者：
WATSON, CW
影响因子：
5.8
作者：
Leng, Ning;Dawson, John A.;Kendziorski, Christina
通讯作者：
Kendziorski, Christina