Experimental Evolution Expands the Breadth of Adaptation to an Environmental Gradient Correlated With Genome Reduction.
Experimental Evolution Expands the Breadth of Adaptation to an Environmental Gradient Correlated With Genome Reduction.
复制标题
DOI：
10.3389/fmicb.2022.826894
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
Ying BW
中科院分区：
文献类型：
--
作者：
Kurokawa M;Nishimura I;Ying BW
适应性进化是否以及如何与基因组协调地调整适应的广度，是将进化与生态学联系起来的基本问题。为了解决这些问题，在五个携带野生型基因组或简化基因组的大肠杆菌菌株中，在定义的最小培养基(C0)中进行了实验进化。随后对祖先和进化的种群进行了跨环境梯度的适应度和化学生态位分析，其中包括最低限度培养基中8种化学成分的29种组合。结果表明，适应不仅针对进化条件(C0)，而且普遍地针对环境梯度，即对8种化学生态位的适应广度扩大。适应性改善的幅度和广度的增加都与基因组减少相关，并且在八个生态位中的两个(即葡萄糖和硫酸盐)中非常显著。直接适应诱导的对环境梯度的相关适应只由几个基因组突变决定。在减少的基因组中，一致地观察到与逐步固定突变相关的适合度的相加增加。总而言之，这项初步调查表明，进化微调了与基因组减少相关的适应的广度。
Whether and how adaptive evolution adjusts the breadth of adaptation in coordination with the genome are essential issues for connecting evolution with ecology. To address these questions, experimental evolution in five Escherichia coli strains carrying either the wild-type genome or a reduced genome was performed in a defined minimal medium (C0). The ancestral and evolved populations were subsequently subjected to fitness and chemical niche analyses across an environmental gradient with 29 combinations of eight chemical components of the minimal medium. The results showed that adaptation was achieved not only specific to the evolutionary condition (C0), but also generally, to the environmental gradient; that is, the breadth of adaptation to the eight chemical niches was expanded. The magnitudes of the adaptive improvement and the breadth increase were both correlated with genome reduction and were highly significant in two out of eight niches (i.e., glucose and sulfate). The direct adaptation-induced correlated adaptation to the environmental gradient was determined by only a few genome mutations. An additive increase in fitness associated with the stepwise fixation of mutations was consistently observed in the reduced genomes. In summary, this preliminary survey demonstrated that evolution finely tuned the breadth of adaptation correlated with genome reduction.
登录
查看更多内容
影响因子：
7.2
作者：
Daubin, Vincent;Szoellosi, Gergely J.
通讯作者：
Szoellosi, Gergely J.
影响因子：
16.6
作者：
Choe, Donghui;Lee, Jun Hyoung;Cho, Byung-Kwan
通讯作者：
Cho, Byung-Kwan
影响因子：
64.8
作者：
Cooper, VS;Lenski, RE
通讯作者：
Lenski, RE
影响因子：
10.7
作者：
Karcagi, Ildiko;Draskovits, Gabor;Papp, Balazs
通讯作者：
Papp, Balazs
影响因子：
10.7
作者：
Barrick JE;Kauth MR;Strelioff CC;Lenski RE
通讯作者：
Lenski RE