Deciphering lignocellulose deconstruction by the white rot fungus Irpex lacteus based on genomic and transcriptomic analyses.
Deciphering lignocellulose deconstruction by the white rot fungus Irpex lacteus based on genomic and transcriptomic analyses.
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基于基因组和转录组分析破译白腐真菌 Irpexlacteus 的木质纤维素解构
DOI：
10.1186/s13068-018-1060-9
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发表时间：
2018
影响因子：
6.3
通讯作者：
Ma F
中科院分区：
文献类型：
--
作者：
Qin X;Su X;Luo H;Ma R;Yao B;Ma F
背景：白腐菌是木质纤维素生物预处理生产二次燃料的最有效的白色腐菌之一。为了阐明木质纤维素降解的分子机制，对I.结果：Irpex lacteusCD 2在9d内可有效降解玉米秸秆中74.9%的木质素、86.3%的纤维素和83.5%的半纤维素。锰过氧化物酶迅速诱导，随后纤维素酶和半纤维素酶的积累。基因组分析表明，I. lacteusCD 2具有一套完整的木质纤维素降解酶系，主要由Ⅱ类过氧化物酶、染料脱色过氧化物酶、辅助酶和182种糖苷水解酶组成。比较转录组学分析证实了选择性降解模式的概念。这些分析还表明，自由基，无论是来自MnP-有机酸的相互作用，或从芬顿反应涉及Fe 2+和H2 O2，可以发挥重要作用，在木质纤维素降解。乳酸菌CD 2与低胞外糖苷酶结合，将植物细胞壁多糖切割成可发酵的糖，可以解释I. lacteus。我们的研究还暗示了自由基对于未来设计新颖的、稳健的木质纤维素降解酶鸡尾酒的重要性。
Background:Irpex lacteusis one of the most potent white rot fungi for biological pretreatment of lignocellulose for second biofuel production. To elucidate the underlying molecular mechanism involved in lignocellulose deconstruction, genomic and transcriptomic analyses were carried out forI. lacteusCD2 grown in submerged fermentation using ball-milled corn stover as the carbon source.Results:Irpex lacteusCD2 efficiently decomposed 74.9% lignin, 86.3% cellulose, and 83.5% hemicellulose in corn stover within 9 days. Manganese peroxidases were rapidly induced, followed by accumulation of cellulase and hemicellulase. Genomic analysis revealed thatI. lacteusCD2 possessed a complete set of lignocellulose-degrading enzyme system composed mainly of class II peroxidases, dye-decolorizing peroxidases, auxiliary enzymes, and 182 glycoside hydrolases. Comparative transcriptomic analysis substantiated the notion of a selection mode of degradation. These analyses also suggested that free radicals, derived either from MnP-organic acid interplay or from Fenton reaction involving Fe2+and H2O2, could play an important role in lignocellulose degradation.Conclusions:The selective strategy employed byI. lacteusCD2, in combination with low extracellular glycosidases cleaving plant cell wall polysaccharides into fermentable sugars, may account for high pretreatment efficiency ofI. lacteus. Our study also hints the importance of free radicals for future designing of novel, robust lignocellulose-degrading enzyme cocktails.
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影响因子：
6.3
作者：
Couturier M;Navarro D;Chevret D;Henrissat B;Piumi F;Ruiz-Dueñas FJ;Martinez AT;Grigoriev IV;Riley R;Lipzen A;Berrin JG;Master ER;Rosso MN
通讯作者：
Rosso MN
影响因子：
14.9
作者：
Grigoriev IV;Nikitin R;Haridas S;Kuo A;Ohm R;Otillar R;Riley R;Salamov A;Zhao X;Korzeniewski F;Smirnova T;Nordberg H;Dubchak I;Shabalov I
通讯作者：
Shabalov I
DOI：
10.1111/j.1757-1707.2009.01004.x
发表时间：
2009-02-18
期刊：
Global change biology. Bioenergy
影响因子：
--
作者：
Dodd D;Cann IK
通讯作者：
Cann IK
影响因子：
5.9
作者：
Garcia-Torreiro, M.;Lopez-Abelairas, M.;Lema, J. M.
通讯作者：
Lema, J. M.
DOI：
10.1073/pnas.1323629111
发表时间：
2014-04-29
影响因子：
11.1
作者：
Agger, Jane W.;Isaksen, Trine;Westereng, Bjorge
通讯作者：
Westereng, Bjorge