A UV-responsive G2 checkpoint in rodent cells
A UV-responsive G2 checkpoint in rodent cells
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啮齿动物细胞中的紫外线响应 G2 检查点
DOI：
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发表时间：
1995
影响因子：
5.3
通讯作者：
V. Bohr
中科院分区：
文献类型：
--
作者：
D. Orren;L. Petersen;V. Bohr
我们研究了紫外线照射对同步中国仓鼠卵巢细胞周期进程的影响。细胞在S期或G2期的同步是通过使用在G1/S边界阻断细胞周期进程的氨莫辛的新方案完成的。去除氨基葡萄糖后，细胞同步通过S期和G2期，允许在任何一个期的特定点操作细胞。G1期细胞的同步是通过一段时间的血清饥饿后释放细胞实现的。通过这些方法同步的细胞在G1、S和G2的特定点进行紫外线照射，并监测它们在细胞周期中的后续进展。紫外线照射g1同步细胞引起剂量依赖性延迟进入S期。在G2中，S期同步细胞的照射抑制了S期的进展，然后导致细胞的积累延长了一段时间。在这一延长的时期，细胞亚群的凋亡被注意到。紫外线照射G2同步细胞时，G2阻滞时间较短。阻滞本身及其持续时间分别取决于照射和紫外线剂量的时间（G2期内）。因此，我们在G2期定义了一个以前未描述过的（在哺乳动物细胞中）紫外线反应检查点。讨论了这些发现对DNA代谢的影响。
We have studied the effect of UV irradiation on the cell cycle progression of synchronized Chinese hamster ovary cells. Synchronization of cells in S or G2 phase was accomplished by the development of a novel protocol using mimosine, which blocks cell cycle progression at the G1/S boundary. After removal of mimosine, cells proceed synchronously through the S and G2 phases, allowing manipulation of cells at specific points in either phase. Synchronization of cells in G1 was achieved by release of cells after a period of serum starvation. Cells synchronized by these methods were UV irradiated at defined points in G1, S, and G2, and their subsequent progression through the cell cycle was monitored. UV irradiation of G1-synchronized cells caused a dose-dependent delay in entry into S phase. Irradiation of S-phase-synchronized cells inhibited progression through S phase and then resulted in accumulation of cells for a prolonged interval in G2. Apoptosis of a subpopulation of cells during this extended period was noted. UV irradiation of G2-synchronized cells caused a shorter G2 arrest. The arrest itself and its duration were dependent upon the timing (within G2 phase) of the irradiation and the UV dose, respectively. We have thus defined a previously undescribed (in mammalian cells) UV-responsive checkpoint in G2 phase. The implications of these findings with respect to DNA metabolism are discussed.
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DOI：
10.1016/0921-8777(89)90014-1
发表时间：
1989
期刊：
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影响因子：
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作者：
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影响因子：
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DOI：
10.1073/pnas.89.22.10696
发表时间：
1992
影响因子：
11.1
作者：
Sweder,KS;Hanawalt,PC
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影响因子：
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影响因子：
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作者：
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通讯作者：
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