Genotoxicity evaluation of locally produced dental porcelain--an in vitro study using the Ames and Comet assays.
Genotoxicity evaluation of locally produced dental porcelain--an in vitro study using the Ames and Comet assays.
复制标题
本地生产的牙瓷的遗传毒性评估——使用艾姆斯和彗星测定的体外研究。
DOI：
10.1016/j.tiv.2009.05.025
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
A. R. Ismail
中科院分区：
文献类型：
--
作者：
M. Noushad;T. Kannan;A. Husein;Haswati Abdullah;A. R. Ismail
本研究的目的是使用艾姆斯和彗星试验确定当地生产的牙科陶瓷（马来西亚Sains大学）的遗传毒性。在艾姆斯试验中，使用了沙门氏菌菌株的4种基因型变体（TA 98、TA 100、TA 1537和TA 1535），这些菌株在几个基因中携带突变。在存在和不存在代谢活化（S9）的情况下，将牙科陶瓷与5种不同剂量的这4种菌株一起孵育，并根据回复突变菌落数评估结果。同时，使用适当的阳性对照品以验证试验。与阴性对照相比，用本地生产的牙科陶瓷处理的每个平板的回复突变菌落的平均数不到两倍。在彗星试验中，用三种不同浓度的牙瓷处理L929（CCL-1 ATCC，USA）小鼠成纤维细胞，同时进行阴性和阳性对照。用于测量DNA损伤的尾矩几乎等于阴性对照的尾矩，表明本地生产的牙科陶瓷没有诱导任何DNA损伤。结果表明，在目前的测试条件下，本地生产的牙科陶瓷是无遗传毒性的。
The aim of this study was to determine the genotoxicity of a locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Ames and Comet assays. In the Ames assay, four genotypic variants of the Salmonella strains (TA98, TA100, TA1537 and TA1535) carrying mutations in several genes were used. The dental porcelain was incubated with these four strains in five different doses both in the presence and absence of metabolic activation (S9) and the result was assessed based on the number of revertant colonies. Concurrently, appropriate positive controls were used so as to validate the test. The average number of revertant colonies per plate treated with locally produced dental porcelain was less than double as compared to that of negative control. In the Comet assay, L929 (CCL-1 ATCC, USA) mouse fibroblast cells were treated with the dental porcelain in three different concentrations along with concurrent negative and positive controls. The tail moment which was used as a measurement of DNA damage was almost equal to that of the negative control, suggesting that the locally produced dental porcelain did not induce any DNA damage. The results indicated that the locally produced dental porcelain is non-genotoxic under the present test conditions.
影响因子：
5
作者：
Eick, JD;Kostoryz, EL;Yourtee, DM
通讯作者：
Yourtee, DM