Bacillus subtilis NrnB is expressed during sporulation and acts as a unique 3'-5' exonuclease.
Bacillus subtilis NrnB is expressed during sporulation and acts as a unique 3'-5' exonuclease.
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DOI：
10.1093/nar/gkad662
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发表时间：
2023-10-13
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
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作者：
所有细胞都采用内切核糖核酸酶和外切核糖核酸酶的组合来将长RNA聚合物降解成长度为2-5个核苷酸的片段。这些短RNA片段被nanoRNase加工成单核糖核苷酸。已显示nanoRNA酶的遗传消耗增加短RNA的丰度。这会不利地影响生存力、毒力和适应性，表明短RNA是代谢负担。以前，我们提供的证据表明NrnA是枯草芽孢杆菌的管家nanoRNase。在此，我们研究了进化相关蛋白B的生物学和生化功能。枯草芽孢杆菌NrnB（NrnBBs）。这些实验表明NrnB与NrnA惊人地不同。NrnA作用于RNA底物的5′端，而NrnB作用于3′端。此外，NrnA在标准生长条件下组成型表达，而NrnB在内生孢子形成期间选择性表达。此外，NrnA仅加工短RNA，而NrnB出乎意料地加工短RNA和较长RNA。事实上，NrnB的诱导型表达甚至可以补充已知的全局3′-5′核糖核酸外切酶的损失，表明它作为一般的核酸外切酶起作用。总之，这些数据表明，NrnB蛋白，这是广泛存在于厚壁菌门，Epsilonproteobacteria和古细菌，是从根本上不同于NrnA蛋白，并可用于专门的目的。
All cells employ a combination of endo- and exoribonucleases to degrade long RNA polymers to fragments 2–5 nucleotides in length. These short RNA fragments are processed to monoribonucleotides by nanoRNases. Genetic depletion of nanoRNases has been shown to increase abundance of short RNAs. This deleteriously affects viability, virulence, and fitness, indicating that short RNAs are a metabolic burden. Previously, we provided evidence that NrnA is the housekeeping nanoRNase for Bacillus subtilis. Herein, we investigate the biological and biochemical functions of the evolutionarily related protein, B. subtilis NrnB (NrnBBs). These experiments show that NrnB is surprisingly different from NrnA. While NrnA acts at the 5′ terminus of RNA substrates, NrnB acts at the 3′ terminus. Additionally, NrnA is expressed constitutively under standard growth conditions, yet NrnB is selectively expressed during endospore formation. Furthermore, NrnA processes only short RNAs, while NrnB unexpectedly processes both short RNAs and longer RNAs. Indeed, inducible expression of NrnB can even complement the loss of the known global 3′-5′ exoribonucleases, indicating that it acts as a general exonuclease. Together, these data demonstrate that NrnB proteins, which are widely found in Firmicutes, Epsilonproteobacteria and Archaea, are fundamentally different than NrnA proteins and may be used for specialized purposes.
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影响因子：
9.9
作者：
Arrieta-Ortiz ML;Hafemeister C;Bate AR;Chu T;Greenfield A;Shuster B;Barry SN;Gallitto M;Liu B;Kacmarczyk T;Santoriello F;Chen J;Rodrigues CD;Sato T;Rudner DZ;Driks A;Bonneau R;Eichenberger P
通讯作者：
Eichenberger P
影响因子：
11.1
作者：
Hui MP;Foley PL;Belasco JG
通讯作者：
Belasco JG
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
14.9
作者：
Fang M;Zeisberg WM;Condon C;Ogryzko V;Danchin A;Mechold U
通讯作者：
Mechold U
DOI：
10.1261/rna.078962.121
发表时间：
2022-03
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Ingle S;Chhabra S;Chen J;Lazarus MB;Luo X;Bechhofer DH
通讯作者：
Bechhofer DH