Homing of Long‐Term and Short‐Term Engrafting Cells In Vivo a
Homing of Long‐Term and Short‐Term Engrafting Cells In Vivo a
复制标题
体内长期和短期移植细胞的归巢
DOI：
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发表时间：
1999
影响因子：
5.2
通讯作者：
S. Sharkis
中科院分区：
文献类型：
--
作者：
S. Lanzkron;M. Collector;S. Sharkis
摘要：可以根据大小将长期再生造血干细胞与提供辐射防护的细胞（短期再生细胞）分开。这可能是长期重新增殖细胞的静态性质的结果。为了定义这些细胞群的活性，我们使用了一种染料 PKH26+，它会融入细胞膜，并在子细胞分裂时平均分配给子细胞。我们能够在移植后在受体的造血组织中检索 PKH26+ 标记的细胞。我们还可以评估它们的细胞周期状态以及它们在有限稀释中重建次级致死辐照宿主的短期和长期能力。结果表明，长期再生细胞在植入后不久在骨髓中保持静止状态，而辐射防护细胞则分裂得更快。我们无法在移植后的外周血中检测到标记细胞，即使细胞归巢于脾脏和骨髓，骨髓中的细胞在重建受到致命辐射的第二宿主方面也明显更有能力。
Abstract: Long‐term repopulating hematopoietic stem cells can be separated from cells which provided radioprotection (short‐term repopulating cells) on the basis of size. This might be a result of the quiescent nature of long‐term repopulating cells. To define the activity of these populations we utilized a dye, PKH26+, which incorporates into the membrane of cells and is equally distributed to daughter cells when they divide. We were able to retrieve PKH26+‐labeled cells posttransplant in the hematopoietic tissues of the recipients. We could also assess their cell cycle status and their ability, short‐and long‐term, to reconstitute secondary lethally irradiated hosts in limiting dilution. The results suggest that long‐term repopulating cells remain quiescent in the bone marrow shortly after engraftment, whereas cells which radioprotect are more rapidly dividing. We could not detect labeled cells in the peripheral blood posttransplant, and even though cells homed to both the spleen and bone marrow the cells in the bone marrow were significantly more competent at reconstituting lethally irradiated secondary hosts.
荧光原位杂交确定小鼠骨髓移植后的植入状态。
影响因子：
--
作者：
Hawkins,AL;Jones,RJ;Zehnbauer,BA;Zicha,MS;Collector,MJ;Sharkis,SJ;Griffin,CA
通讯作者：
Griffin,CA
DOI：
10.1073/pnas.92.21.9647
发表时间：
1995-10-10
影响因子：
11.1
作者：
PAPAYANNOPOULOU, T;CRADDOCK, C;WOLF, NS
通讯作者：
WOLF, NS
影响因子：
56.9
作者：
BERARDI, AC;WANG, AL;SCADDEN, DT
通讯作者：
SCADDEN, DT