Mineral Surface Chemistry and Nanoparticle-aggregation Control Membrane Self-Assembly
Mineral Surface Chemistry and Nanoparticle-aggregation Control Membrane Self-Assembly
复制标题
矿物表面化学和纳米颗粒聚集控制膜自组装
作者：
Nita Sahai;Hussein Kaddour;Punam Dalai;Ziqiu Wang;Garrett F. Bass;Min Gao
脂质双层膜的自组装以包围功能性生物分子，从而定义了“原始细胞”，是地球上生命出现的一个开创性时刻，很可能发生在矿泉水界面的微环境中。矿物质-脂质相互作用也与生物医学、工业和技术过程相关。然而，尚未发现结构活性关系（SAR）可以预测矿物表面的脂质自组装。在这里，我们研究了矿物质对作为模型原始细胞膜的单链两亲物组成的囊泡的自组装和存活的影响。在高负载量 (mg/mL) 的带正电纳米颗粒矿物质存在下，表观临界囊泡浓度 (CVC) 增加，表明在这种情况下膜自组装不利。高于 CVC，在矿物质存在的情况下，初始囊泡形成速率更快。速率与矿物的等电点 (IEP) 和反应表面积相关。 IEP 取决于晶体结构、化学成分和表面水合。因此，膜自组装表现出对基本矿物特性的合理依赖。一旦形成，膜的渗透性（完整性）就不受矿物质的影响。这表明原始细胞可能在岩石表面存活。这些SAR可能有助于预测早期地球和其他岩石行星上原始细胞膜的形成和生存，以及各种其他现象中的两亲矿物相互作用。
The self-assembly of lipid bilayer membranes to enclose functional biomolecules, thus defining a “protocell,” was a seminal moment in the emergence of life on Earth and likely occurred at the micro-environment of the mineral-water interface. Mineral-lipid interactions are also relevant in biomedical, industrial and technological processes. Yet, no structure-activity relationships (SARs) have been identified to predict lipid self-assembly at mineral surfaces. Here we examined the influence of minerals on the self-assembly and survival of vesicles composed of single chain amphiphiles as model protocell membranes. The apparent critical vesicle concentration (CVC) increased in the presence of positively-charged nanoparticulate minerals at high loadings (mg/mL) suggesting unfavorable membrane self-assembly in such situations. Above the CVC, initial vesicle formation rates were faster in the presence of minerals. Rates were correlated with the mineral’s isoelectric point (IEP) and reactive surface area. The IEP depends on the crystal structure, chemical composition and surface hydration. Thus, membrane self-assembly showed rational dependence on fundamental mineral properties. Once formed, membrane permeability (integrity) was unaffected by minerals. Suggesting that, protocells could have survived on rock surfaces. These SARs may help predict the formation and survival of protocell membranes on early Earth and other rocky planets, and amphiphile-mineral interactions in diverse other phenomena.
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影响因子：
3.4
作者：
Chen, IA;Szostak, JW
通讯作者：
Szostak, JW
T 淋巴细胞 Jurkat 系的氧化物依赖性粘附。
DOI：
10.1021/la8040192
发表时间：
2009
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Stevens,MJ;Donato,LeslieJ;Lower,StevenK;Sahai,Nita
通讯作者：
Sahai,Nita
矿物表面化学和毒性是否有助于微生物细胞外聚合物的进化？
影响因子：
4.2
作者：
Xu,Jie;Campbell,JayM;Zhang,Nianli;Hickey,WilliamJ;Sahai,Nita
通讯作者：
Sahai,Nita
DOI：
10.1073/pnas.0308045101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Chen, IA;Szostak, JW
通讯作者：
Szostak, JW