First complete-genome documentation of HIV-1 intersubtype superinfection with transmissions of diverse recombinants over time to five recipients.
First complete-genome documentation of HIV-1 intersubtype superinfection with transmissions of diverse recombinants over time to five recipients.
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首个 HIV-1 亚型间重复感染的完整基因组记录，随着时间的推移，不同重组体会传播给 5 名接受者
DOI：
10.1371/journal.ppat.1009258
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发表时间：
2021-03
期刊：
影响因子：
6.7
通讯作者：
Han X
中科院分区：
文献类型：
--
作者：
Gao Y;He S;Tian W;Li D;An M;Zhao B;Ding H;Xu J;Chu Z;Shang H;Han X
人类免疫缺陷病毒1型（human immunodeficiency virus type 1，HIV-1）重组体是由HIV-1不同毒株在合并感染或重叠感染时发生重组而产生的。然而，缺乏直接证据支持HIV-1重组体从合并感染/重叠感染的供体传播到假定的受体。在这里，我们报告的起源和进化之间的关系，一组重组从CRF01_AE/CRF07_BC超感染的假定供体和不同的CRF01_AE/CRF07_BC重组从5个假定的收件人。对这6名男男性行为者的社会人口学特征和性行为进行访谈，发现他们的择偶方式相似：交友网站和社交圈。系统发育分析和重组分析表明，6例患者的近全长基因组序列在最大似然系统发育树中形成了一个不同于已知HIV-1基因型的单系簇，均由CRF01_AE和CRF07_BC片段组成，在env上有两个共同的断点，彼此共有4-7个断点。此外，5个受体的重组菌株的3'半基因组与来自重叠感染供体的纵向样品中的菌株具有相同/相似的重组结构。在最大分支可信度树中，来自供体的杂交种为并系，而5个受体为单系或多系。Bayesian分析证实，供体CRF01_AE和CRF07_BC菌株的最新共同祖先（tMRCA）时间分别为2009.2和2010.7，均早于5个受体的重组子出现。我们的结果表明，密切相关的独特的重组形式的HIV-1可能是后代的一系列重组逐渐产生的重叠感染的病人。这一发现强调了早期开始抗逆转录病毒治疗以及追踪和检测多重HIV-1感染患者的伴侣的重要性。病毒复制是人类免疫缺陷病毒1型（HIV-1）快速进化和多样化的主要机制。多重HIV-1感染被认为是产生新的重组毒株的先决条件。共享一些断点的突变体可能有共同的祖先或直接的父母/祖先关系，或者在进化中不相关。然而，缺乏证据支持HIV-1重组体从合并感染/重叠感染的供体传播到具有重组后代的假定受体。在这里，我们报告了一组HIV-1 CRF01_AE/CRF07_BC独特的重组形式（URF）从6个HIV-1感染的男性有性行为（MSM）在中国具有相似的重组结构和同源亲本株。此外，来自5个受体的所有重组体的3'半基因组与先前的一个重叠感染供体的连续样品具有相似或相同的形式，并且在最大分支可信度树中呈现并系-单系或并系-多系关系。这些数据表明，HIV-1重叠感染可能是一系列密切相关的HIV-1 CRF01_AE/CRF07_BC URF的潜在传播源。我们的研究强调了持续监测和早期启动抗逆转录病毒治疗的重要性，在HIV-1重叠感染的个人与积极的高危行为和药物滥用，以防止重组株的产生和传播的HIV感染者。
Human immunodeficiency virus type 1 (HIV-1) recombinants in the world are believed to be generated through recombination between distinct HIV-1 strains among coinfection or superinfection cases. However, direct evidence to support transmission of HIV-1 recombinants from a coinfected/superinfected donor to putative recipient is lacking. Here, we report on the origin and evolutionary relationship between a set of recombinants from a CRF01_AE/CRF07_BC superinfected putative donor and diverse CRF01_AE/CRF07_BC recombinants from five putative recipients. Interviews on sociodemographic characteristics and sexual behaviors for these six HIV-1-infected men who have sex with men showed that they had similar ways of partner seeking: online dating sites and social circles. Phylogenetic and recombination analyses demonstrated that the near-full-length genome sequences from six patients formed a monophyletic cluster different from known HIV-1 genotypes in maximum likelihood phylogenetic trees, were all composed of CRF01_AE and CRF07_BC fragments with two common breakpoints on env, and shared 4–7 breakpoints with each other. Moreover, 3’ half-genomes of recombinant strains from five recipients had identical/similar recombinant structures with strains at longitudinal samples from the superinfected donor. Recombinants from the donor were paraphyletic, whereas five recipients were monophyletic or polyphyletic in the maximum clade credibility tree. Bayesian analyses confirmed that the estimated time to the most recent common ancestor (tMRCA) of CRF01_AE and CRF07_BC strains of the donor was 2009.2 and 2010.7, respectively, and all were earlier than the emergence of recombinants from five recipients. Our results demonstrated that the closely related unique recombinant forms of HIV-1 might be the descendent of a series of recombinants generated gradually in a superinfected patient. This finding highlights the importance of early initiation of antiretroviral therapy as well as tracing and testing of partners in patients with multiple HIV-1 infection. Recombination is a major mechanism for rapid evolution and diversification of human immunodeficiency virus type 1 (HIV-1). Multiple HIV-1 infection is believed to be a prerequisite for generating new recombinant strains. Recombinants sharing some breakpoints might have a common ancestor or direct parental/progenitor relationship or be irrelevant in evolution. However, evidence to support transmission of HIV-1 recombinants from coinfected/superinfected donor to putative recipients with recombinant descendants is lacking. Here, we report on a set of HIV-1 CRF01_AE/CRF07_BC unique recombinant forms (URFs) from six HIV-1-infected men who have sex with men (MSM) in China with similar recombination structures and homologous parental strains. Moreover, the 3’ half-genomes of all recombinants from five recipients had similar or identical forms with earlier continuous samples of a superinfected donor, and presented paraphyletic-monophyletic or paraphyletic-polyphyletic relationships in maximum clade credibility trees. These data suggest that HIV-1 superinfection might be a potential source of transmission of a series of closely related HIV-1 CRF01_AE/CRF07_BC URFs. Our study emphasizes the importance of continuous surveillance and early initiation of antiretroviral therapy in HIV-1-superinfected individuals with active high-risk behavior and substance abuse to prevent the generation and spread of recombinant strains among HIV-infected individuals.
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影响因子：
12.7
作者：
Konings, FAJ;Burda, ST;Nyambi, PN
通讯作者：
Nyambi, PN
影响因子：
4.8
作者：
Jia L;Li L;Gui T;Liu S;Li H;Han J;Guo W;Liu Y;Li J
通讯作者：
Li J
DOI：
10.1128/genomea.00050-12
发表时间：
2013-01
期刊：
Genome announcements
影响因子：
--
作者：
Han X;An M;Zhang W;Cai W;Chen X;Takebe Y;Shang H
通讯作者：
Shang H
影响因子：
3.7
作者：
Han X;An M;Zhao B;Duan S;Yang S;Xu J;Zhang M;McGoogan JM;Takebe Y;Shang H
通讯作者：
Shang H
影响因子：
4.8
作者：
Galetto, R;Moumen, A;Negroni, M
通讯作者：
Negroni, M