GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.
GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.
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GNG5是一种与神经胶质瘤细胞迁移、增殖和不良预后相关的新型癌基因
DOI：
10.1186/s12935-021-01935-7
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发表时间：
2021-06-07
影响因子：
5.8
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Zhang W;Liu Z;Liu B;Jiang M;Yan S;Han X;Shen H;Na M;Wang Y;Ren Z;Liu B;Jiang Z;Gao Y;Lin Z
尽管已经报道了许多用于检测胶质瘤的生物标志物，但该疾病的预后仍然很差，因此，需要鉴定新的生物标志物。GNG 5是G蛋白家族的一部分，与不同的恶性肿瘤有关，但GNG 5在胶质瘤中的作用尚未研究。因此，我们的目的是确定GNG 5与胶质瘤预后的关系，并确定一个新的生物标志物，用于诊断和治疗胶质瘤。我们使用了来自多个数据库和临床数据的一千多个胶质瘤的数据来确定GNG 5在胶质瘤中的表达。基于临床数据和CGGA数据库，我们使用各种分析方法确定了GNG 5与多种分子和临床特征以及预后的相关性。通过共表达分析和GSEA检测胶质瘤中GNG 5相关基因及其可能的信号通路。用ESTIMATE、ssGSEA和TIMER检测GNG 5与免疫微环境的关系。通过功能实验探讨GNG 5在胶质瘤细胞中的功能。GNG 5在胶质瘤中高表达，其表达水平与病理分级、组织学类型、年龄、肿瘤复发呈正相关，与异柠檬酸脱氢酶突变、1 p/19共缺失、化疗呈负相关。此外，GNG 5作为独立的危险因素与总生存时间呈负相关。GSEA揭示了GNG 5在胶质瘤中功能的潜在信号通路，包括细胞粘附分子信号通路。基于ssGSEA、ESTIMATE和TIMER的分析表明GNG 5表达与胶质瘤中的各种免疫细胞之间存在相关性。体内外实验表明，GNG 5可参与胶质瘤细胞的增殖和迁移。基于大数据平台和使用不同的数据库来证实使用各种数据集以及体外和体内实验获得的结果，我们的研究首次揭示了GNG 5作为一种癌基因在胶质瘤中过表达，并且可以抑制胶质瘤细胞的增殖和迁移，导致患者预后不良。因此，GNG 5是一个潜在的新的生物标志物，用于临床诊断和治疗胶质瘤。在线版本包含补充材料，可通过10.1186/s12935-021-01935-7获得。
Although many biomarkers have been reported for detecting glioma, the prognosis for the disease remains poor, and therefore, new biomarkers need to be identified. GNG5, which is part of the G-protein family, has been associated with different malignant tumors, though the role of GNG5 in glioma has not been studied. Therefore, we aimed to identify the relationship between GNG5 and glioma prognosis and identify a new biomarker for the diagnosis and treatment of gliomas. We used data on more than a thousand gliomas from multiple databases and clinical data to determine the expression of GNG5 in glioma. Based on clinical data and CGGA database, we identified the correlation between GNG5 and multiple molecular and clinical features and prognosis using various analytical methods. Co-expression analysis and GSEA were performed to detect GNG5-related genes in glioma and possible signaling pathways involved. ESTIMATE, ssGSEA, and TIMER were used to detect the relationship between GNG5 and the immune microenvironment. Functional experiments were performed to explore the function of GNG5 in glioma cells. GNG5 is highly expressed in gliomas, and its expression level is positively correlated with pathological grade, histological type, age, and tumor recurrence and negatively correlated with isocitrate dehydrogenase mutation, 1p/19 co-deletion, and chemotherapy. Moreover, GNG5 as an independent risk factor was negatively correlated with the overall survival time. GSEA revealed the potential signaling pathways involved in GNG5 function in gliomas, including cell adhesion molecules signaling pathway. The ssGSEA, ESTIMATE, and TIMER based analysis indicated a correlation between GNG5 expression and various immune cells in glioma. In vivo and in vitro experiments showed that GNG5 could participate in glioma cell proliferation and migration. Based on the large data platform and the use of different databases to corroborate results obtained using various datasets, as well as in vitro and in vivo experiments, our study reveals for the first time that GNG5, as an oncogene, is overexpressed in gliomas and can inhibit the proliferation and migration of glioma cells and lead to poor prognosis of patients. Thus, GNG5 is a potential novel biomarker for the clinical diagnosis and treatment of gliomas. The online version contains supplementary material available at 10.1186/s12935-021-01935-7.
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影响因子：
3.6
作者：
Dimitrov L;Hong CS;Yang C;Zhuang Z;Heiss JD
通讯作者：
Heiss JD
影响因子：
3.5
作者：
Chen, Linbo;Lu, Dewen;Xu, Feng
通讯作者：
Xu, Feng
DOI：
10.1159/000134059
发表时间：
1995-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
AHMAD, W;LI, S;ARONSON, NN
通讯作者：
ARONSON, NN
DOI：
10.18632/aging.101415
发表时间：
2018-04-16
期刊：
Aging
影响因子：
--
作者：
Jia D;Li S;Li D;Xue H;Yang D;Liu Y
通讯作者：
Liu Y
JAK1作为乳腺癌的预后标志物及其与免疫浸润的相关性
影响因子：
5.2
作者：
Chen, Bo;Lai, Jianguo;Liao, Ning
通讯作者：
Liao, Ning