Different expression of Sox9 and Runx2 between chondrosarcoma and dedifferentiated chondrosarcoma cell line
Different expression of Sox9 and Runx2 between chondrosarcoma and dedifferentiated chondrosarcoma cell line
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软骨肉瘤与去分化软骨肉瘤细胞系中Sox9和Runx2表达的差异
DOI：
10.1097/cej.0b013e32833d942f
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发表时间：
2010-11
影响因子：
2.4
通讯作者：
Tang, Guoqing
中科院分区：
文献类型：
--
作者：
Zhao, Hui;Ren, Tingting;Zhao, Fulong;Guo, Wei;Tang, Xiaodong;Lu, Xinchang;Tang, Guoqing
去分化软骨肉瘤（DDCS）是软骨肉瘤（CCS）的高度恶性变体，其特征是高级别肉瘤毗邻低级别软骨样肿瘤。由于其复杂的组成，该肿瘤的组织发生仍存在争议。早些时候，我们利用软骨肉瘤组织进行了DNA微阵列分析，发现与DDCS相比，Sox9和runt相关转录因子2（Runx2）在CCS中存在差异表达。在这里，我们使用逆转录聚合酶链反应、蛋白质印迹和免疫细胞化学分析了 NDCS-1 (DDCS) 和 SW1353 (CCS) 细胞系中的 Sox9、Runx2、Col2a1 和 Col1a1。结果显示NDCS-1细胞中Runx2和Col1a1高表达，Sox9和Col2a1低表达。然而，在 SW1353 细胞中，基因表达发生了逆转。这些发现证明Sox9和Runx2参与了DDCS的发生和发展。
Dedifferentiated chondrosarcoma (DDCS), a highly malignant variant of chondrosarcoma (CCS), is characterized by high-grade sarcoma adjacent to low-grade chondroid tumor. Owing to its complicated composition, the histogenesis of this tumor remains controversial. Earlier, we carried out DNA microarray analysis using chondrosarcomatous tissues, and found that Sox9 and runt-related transcription factor 2 (Runx2) were differentially expressed in CCS compared with DDCS. Here, we analyzed Sox9, Runx2, Col2a1, and Col1a1 in NDCS-1 (DDCS) and SW1353 (CCS) cell lines using reverse transcriptase-polymerase chain reaction, western blot, and immunocytochemistry. The results showed high expression of Runx2 and Col1a1, and low expression of Sox9 and Col2a1 in NDCS-1 cells. In SW1353 cells, however, gene expressions were reversed. These findings provide evidence that Sox9 and Runx2 are involved in the occurrence and development of DDCS.
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影响因子：
6.2
作者：
Staals, Eric L.;Bacchini, Patrizia;Bertoni, Franco
通讯作者：
Bertoni, Franco
影响因子：
10.5
作者：
Akiyama, H;Chaboissier, MC;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
DOI：
10.32388/qpw17u
发表时间：
2020-02
期刊：
Definitions
影响因子：
--
作者：
Andy Langdown
通讯作者：
Andy Langdown
影响因子：
6.2
作者：
D. Dahlin;J. Beabout
通讯作者：
D. Dahlin;J. Beabout
影响因子：
4.8
作者：
Kou, I;Ikegawa, S
通讯作者：
Ikegawa, S