Multianalyte serology in home-sampled blood enables an unbiased assessment of the immune response against SARS-CoV-2.
Multianalyte serology in home-sampled blood enables an unbiased assessment of the immune response against SARS-CoV-2.
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DOI：
10.1038/s41467-021-23893-4
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发表时间：
2021-06-17
影响因子：
16.6
通讯作者：
Schwenk JM
中科院分区：
文献类型：
--
作者：
Roxhed N;Bendes A;Dale M;Mattsson C;Hanke L;Dodig-Crnković T;Christian M;Meineke B;Elsässer S;Andréll J;Havervall S;Thålin C;Eklund C;Dillner J;Beck O;Thomas CE;McInerney G;Hong MG;Murrell B;Fredolini C;Schwenk JM
血清学检测对于遏制 COVID-19 大流行的后果至关重要。然而，大多数测定仍然仅限于在医疗保健范围内收集的单一分析物和样本。因此，我们建立了一种多分析物和多重方法，可以根据家庭采样的干血斑 (DBS) 中多个版本的 SARS-CoV-2 蛋白（S、RBD、N）可靠地分析 IgG 和 IgM 水平。我们分析了 2020 年春季从瑞典斯德哥尔摩人群中随机收集的 878 名未确诊个体的 DBS，并使用分类方法估计累积血清阳性率为 12.5%（95% CI：10.3%–14.7%）。其中 5.4% 的样本是针对多种 SARS-CoV-2 蛋白的 IgG+IgM+，2.1% 的样本是针对病毒 S 蛋白的 IgG−IgM+，5.0% 的样本是 IgG+IgM−。与仅 S 蛋白 IgG+ 的受试者相比，多种 SARS-CoV-2 蛋白被归类为 IgG+ 的受试者更频繁地报告流感样症状 (OR = 6.1；p < 0.001)。在所有血清反应呈阳性的病例中，30%是无症状的。我们的策略能够对家庭血液样本中的抗体进行准确的个体水平和多重评估，帮助我们了解针对冠状病毒的免疫反应的未诊断血清阳性率和多样性。在这里，Roxhed 等人。开发一种多重方法来筛查家庭采样的干血斑中针对几种 SARS-CoV-2 蛋白的 IgG 和 IgM 水平，并估计 2020 年春季斯德哥尔摩的血清阳性率为 12.5%。
Serological testing is essential to curb the consequences of the COVID-19 pandemic. However, most assays are still limited to single analytes and samples collected within healthcare. Thus, we establish a multianalyte and multiplexed approach to reliably profile IgG and IgM levels against several versions of SARS-CoV-2 proteins (S, RBD, N) in home-sampled dried blood spots (DBS). We analyse DBS collected during spring of 2020 from 878 random and undiagnosed individuals from the population in Stockholm, Sweden, and use classification approaches to estimate an accumulated seroprevalence of 12.5% (95% CI: 10.3%–14.7%). This includes 5.4% of the samples being IgG+IgM+ against several SARS-CoV-2 proteins, as well as 2.1% being IgG−IgM+ and 5.0% being IgG+IgM− for the virus’ S protein. Subjects classified as IgG+ for several SARS-CoV-2 proteins report influenza-like symptoms more frequently than those being IgG+ for only the S protein (OR = 6.1; p < 0.001). Among all seropositive cases, 30% are asymptomatic. Our strategy enables an accurate individual-level and multiplexed assessment of antibodies in home-sampled blood, assisting our understanding about the undiagnosed seroprevalence and diversity of the immune response against the coronavirus. Here, Roxhed et al. develop a multiplexed approach to screen IgG and IgM levels against several SARS-CoV-2 proteins in home-sampled dried blood spots and estimate seroprevalence of 12.5% in Stockholm in spring of 2020.
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影响因子：
4.2
作者：
Moat SJ;Schulenburg-Brand D;Lemonde H;Bonham JR;Weykamp CW;Mei JV;Shortland GS;Carling RS
通讯作者：
Carling RS
DOI：
10.1074/mcp.m112.026757
发表时间：
2013-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Ayoglu B;Häggmark A;Khademi M;Olsson T;Uhlén M;Schwenk JM;Nilsson P
通讯作者：
Nilsson P
影响因子：
5.2
作者：
Doehla, M.;Boesecke, C.;Streeck, H.
通讯作者：
Streeck, H.
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
影响因子：
5
作者：
Meyer B;Drosten C;Müller MA
通讯作者：
Müller MA