Metabolism, transfer and storage of 5-hydroxytryptamine in blood platelets.
Metabolism, transfer and storage of 5-hydroxytryptamine in blood platelets.
复制标题
血小板中 5-羟色胺的代谢、转移和储存。
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
A. Pletscher
中科院分区：
文献类型：
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作者：
A. Pletscher
近年来，有证据表明芳香族单胺，例如儿茶酚胺和5-羟色胺（5HT），可能在中枢神经系统中充当神经体液递质，因此了解它们的转移、储存和代谢非常重要。然而，对这些过程的直接研究，例如在大脑的孤立突触体中，是有问题的，因为这些元素必须通过相当粗糙的方法（例如，脑匀浆的密度梯度离心）从其自然环境和连接中分离出来。含有大量 5HT 的血小板似乎是更合适的模型，尽管胺在这种情况下的功能尚不清楚。因此，可以在体外相对生理条件下（例如，在血浆或人工缓冲系统中）研究血小板，并且它们还具有代谢、转移和储存5HT的能力。本文报告了该实验室关于血小板中 5HT 的代谢、转移和储存的一些最新发现。根据通常的程序（Bartholini，Pletscher＆Gey，1961）分离包括人类在内的各种物种的血小板，并且在大多数实验中在缓冲液（例如Tyrode）中孵育，使用乙二胺四乙酸二钠来防止血小板聚集。使用血浆作为培养介质的实验与使用 Tyrode 的实验没有显着差异。
In recent years, evidence has been obtained that aromatic monoamines-for example, catecholamines and 5-hydroxytryptamine (5HT)-might function as neurohumoral transmitters in the central nervous system, and knowledge of their transfer, storage and metabolism is therefore important. Direct investigations of these processes, for example, in isolated synaptosomes of the brain, are, however, problematic since these elements have to be separated from their natural environment and connexions by rather crude methods (for example, density gradient centrifugation of brain homogenates). Blood platelets which contain substantial amounts of 5HT seem to be more appropriate models in spite of the fact that the function of the amine in this situation is not well understood. Thus, the platelets can be studied in relatively physiological conditions in vitro-for example, in plasma or artificial buffer systems-and they also have the capacity to metabolize, transfer and store 5HT. Some recent findings in this laboratory concerning the metabolism, transfer and storage of 5HT in blood platelets are reported here. Platelets of various species including man were isolated according to the usual procedures (Bartholini, Pletscher & Gey, 1961) and in most of the experiments incubated in buffers such as Tyrode using disodium-ethylenediaminetetraacetate to prevent platelet aggregation. Experiments with plasma as an incubation medium did not differ markedly from those with Tyrode.
影响人体血小板吸收血清素的因素。
影响因子：
3.4
作者：
Codd,EE;McAllister,TW;Walker,RF
通讯作者：
Walker,RF