Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment.
Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment.
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DOI：
10.1038/s42003-020-01462-7
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发表时间：
2020-12-04
影响因子：
5.9
通讯作者：
Weinstein BM
中科院分区：
文献类型：
--
作者：
Stratman AN;Burns MC;Farrelly OM;Davis AE;Li W;Pham VN;Castranova D;Yano JJ;Goddard LM;Nguyen O;Galanternik MV;Bolan TJ;Kahn ML;Mukouyama YS;Weinstein BM
在早期发育过程中，血管平滑肌细胞（vSMC）在动脉上相对于静脉上的优先积累是一种被充分描述的现象，但这种极化背后的分子途径还没有得到很好的理解。在斑马鱼中，cxcr 4a受体（哺乳动物CXCR 4）及其配体cxcl 12 b（哺乳动物CXCL 12）在与血管发育期间第一个vSMC的到达和分化一致的时间点都优先在动脉上表达。我们发现，自分泌CXCL 12 B/CXCR 4活性导致血管平滑肌细胞的趋化配体PDGFb的生产增加，在体外内皮细胞和增加表达的PDGFb的斑马鱼和小鼠的动脉在体内。此外，我们表明，表达的血流调节转录因子klf 2a在原始静脉负调节cxcr 4/cxcl 12和pdgfb的表达，限制血管平滑肌细胞招聘到动脉血管。总之，该信号传导轴导致在klf 2a表达低且cxcr 4a和pdgfb共表达的位点（即，早期发育期间的动脉）处的vSMC的差异获取。Stratman等人提供了将内皮细胞中的cxcl 12 B/cxcr 4a信号传导轴与血小板衍生生长因子B释放增加联系起来的证据，导致平滑肌细胞向发育中的动脉募集。在早期发育过程中，血流调节转录因子klf 2a的高表达抑制了静脉内皮中的信号传导轴。
The preferential accumulation of vascular smooth muscle cells (vSMCs) on arteries versus veins during early development is a well-described phenomenon, but the molecular pathways underlying this polarization are not well understood. In zebrafish, the cxcr4a receptor (mammalian CXCR4) and its ligand cxcl12b (mammalian CXCL12) are both preferentially expressed on arteries at time points consistent with the arrival and differentiation of the first vSMCs during vascular development. We show that autocrine cxcl12b/cxcr4 activity leads to increased production of the vSMC chemoattractant ligand pdgfb by endothelial cells in vitro and increased expression of pdgfb by arteries of zebrafish and mice in vivo. Additionally, we demonstrate that expression of the blood flow-regulated transcription factor klf2a in primitive veins negatively regulates cxcr4/cxcl12 and pdgfb expression, restricting vSMC recruitment to the arterial vasculature. Together, this signalling axis leads to the differential acquisition of vSMCs at sites where klf2a expression is low and both cxcr4a and pdgfb are co-expressed, i.e. arteries during early development. Stratman et al. provide evidence linking the cxcl12b/cxcr4a signaling axis in endothelial cells to an increased release of platelet-derived growth factor b, leading to the recruitment of smooth muscle cells to developing arteries. This signalling axis is suppressed in the venous endothelium during early development by the high expression of blood flow-regulated transcription factor klf2a.
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DOI：
10.1016/j.carpath.2012.06.006
发表时间：
2013-01
期刊：
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
影响因子：
--
作者：
Gimbrone MA Jr;García-Cardeña G
通讯作者：
García-Cardeña G
影响因子：
20.3
作者：
Dekker, RJ;van Soest, S;Horrevoets, AJG
通讯作者：
Horrevoets, AJG
影响因子：
4.6
作者：
Corti, Paola;Young, Sarah;Roman, Beth L.
通讯作者：
Roman, Beth L.
影响因子：
8.8
作者：
Chen X;Gays D;Milia C;Santoro MM
通讯作者：
Santoro MM
影响因子：
20.1
作者：
Castro, Marco;Lavina, Barbara;Gaengel, Konstantin
通讯作者：
Gaengel, Konstantin