Acceleration of experimental diabetic retinopathy in the rat by omega-3 fatty acids
Acceleration of experimental diabetic retinopathy in the rat by omega-3 fatty acids
复制标题
omega-3 脂肪酸加速大鼠实验性糖尿病视网膜病变
作者：
H. Hammes;A. Weiss;D. Führer;H. Krämer;C. Papavassilis;F. Grimminger
摘要Omega-3脂肪酸对糖尿病视网膜病变的易感因素有几种重要的生物学作用。潜在的发病机制包括血小板功能障碍、改变的类花生酸产生、与受损的细胞变形性和病理性白细胞/内皮相互作用相关的血液粘度增加。因此，我们测试了为期6个月的鱼油（750 mg Maxepa，每周5次），含有14%的二十碳五烯酸（EPA）和10%的二十二碳六烯酸，是否可以抑制链脲佐菌素糖尿病大鼠实验性视网膜病变的发展。鱼油补充剂的有效性通过测量总的、血浆和膜脂肪酸中的EPA浓度以及通过测量脂质介质（白三烯和血栓烷）的产生来评估。定量分析视网膜消化制剂的周细胞损失和无细胞毛细血管的形成。对糖尿病大鼠给予Omega-3脂肪酸导致总脂肪酸中EPA 20∶5增加两倍，血栓烷2/3比率从600（未治疗的糖尿病大鼠）降低至50（治疗的糖尿病大鼠）。尽管有这些生化变化，但糖尿病相关的周细胞损失仍不受影响，鱼油治疗的糖尿病组中无细胞闭塞毛细血管的形成增加了75%（115.1±26.8;未治疗的糖尿病65.2±15.0无细胞毛细血管节段/mm 2视网膜面积）。我们从这项研究中得出结论，膳食鱼油补充剂可能对糖尿病视网膜微血管有害。
SummaryOmega-3 fatty acids exert several important biological effects on factors that may predispose to diabetic retinopathy. Potential pathogenetic mechanisms include platelet dysfunction, altered eicosanoid production, increased blood viscosity in association with impaired cell deformability and pathologic leucocyte/endothelium interaction. Therefore, we tested whether a 6-month administration of fish oil (750 mg Maxepa, 5 times per week), containing 14% eicosapentaenoic acid (EPA) and 10% docosahexaenic acid, could inhibit the development of experimental retinopathy of the streptozotocin-diabetic rat. The efficiency of fish oil supplementation was evaluated by measuring EPA concentrations in total, plasma and membrane fatty acids and by measuring the generation of lipid mediators (leukotrienes and thromboxanes). Retinal digest preparations were quantitatively analysed for pericyte loss, and the formation of acellular capillaries. Omega-3 fatty acid administration to diabetic rats resulted in a twofold increase of EPA 20∶5 in total fatty acids, and a reduction of the thromboxane2/3 ratio from 600 (untreated diabetic rats) to 50 (treated diabetic rats). Despite these biochemical changes, diabetes-associated pericyte loss remained unaffected and the formation of acellular, occluded capillaries was increased by 75% in the fish oil treated diabetic group (115.1±26.8; untreated diabetic 65.2±15.0 acellular capillary segments/mm2 of retinal area). We conclude from this study that dietary fish oil supplementation may be harmful for the diabetic microvasculature in the retina.
影响因子：
39.2
作者：
GLAUBER, H;WALLACE, P;BRECHTEL, G
通讯作者：
BRECHTEL, G
脂肪酸介导的对培养内皮细胞中谷胱甘肽氧化还原循环的影响。
DOI：
10.1093/ajcn/59.1.60
发表时间：
1994
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Toborek,M;Hennig,B
通讯作者：
Hennig,B
DOI：
10.1073/pnas.91.16.7742
发表时间：
1994-08-02
影响因子：
11.1
作者：
WAUTIER, JL;WAUTIER, MP;STERN, D
通讯作者：
STERN, D