Characterization of the complete genome of chikungunya in Zhejiang, China, using a modified virus discovery method based on cDNA-AFLP.
Characterization of the complete genome of chikungunya in Zhejiang, China, using a modified virus discovery method based on cDNA-AFLP.
复制标题
DOI：
10.1371/journal.pone.0083014
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Sun Y;Yan J;Mao H;Zhang L;Lyu Q;Wu Z;Zheng W;Feng C;Zhang Y
基孔肯雅病毒（CHIK）是一种蚊媒新发病原体，在世界范围内，特别是在热带地区，对卫生构成巨大挑战。本文报道了一株新检测到的CHIK菌株，浙江/ CHIK -sy/2012，该菌株来自中国CHIK的非原属地，采用了基于经典dna - aflp的改进方法。然后，我们进行了病因学和系统发育分析，以更好地了解其分子特征和系统发育模式，并有助于进一步评估其在东南亚的持久性。通过这种改进的程序，我们首次确定了基孔肯雅病毒浙江/chik-sy/2012株的全基因组序列，该株于2012年从中国浙江的一名患者中分离出来。序列分析显示，该阳性单链RNA长12,017 bp。在A226V、D284E和A316V中未发现单个氨基酸突变。系统发育分析表明，该菌株与台湾分离的一株菌株具有最大的同源性，该菌株来源于印度尼西亚的一株菌株。chk -sy/2012与以前在中国发现的其他chk病毒属于不同的分支。2012年，利用改进的dna - aflp技术从中国浙江的一例中国发热患者中分离到首个CHIK病毒和首个完整的CHIK -sy/2012病毒基因组序列。该感染与2008年和2010年在中国广东发现的病毒显示出很大的系统发育距离，因为它们来自另一个进化谱系。需要更多的分子流行病学数据来进一步了解、监测和评价中国的CHIK。
Chikungunya (CHIK) virus is a mosquito-borne emerging pathogen presenting great health challenges worldwide, particularly in tropical zones. Here we report a newly detected strain of CHIK, Zhejiang/chik-sy/2012, in China, a nonindigenous region for CHIK, using a modified approach based on the classic cDNA-AFLP. We then performed etiological and phylogenetic analyses to better understand its molecular characterization and phylogenetic pattern, and also to aid in further evaluating its persistence in Southeast Asia. By using this modified procedure, we determined for the first time the complete genome sequence of the chikungunya virus strain, Zhejiang/chik-sy/2012, isolated in 2012 from a patient in Zhejiang, China. Sequence analyses revealed that this positive single strand of RNA is 12,017 bp long. We found no single amino acid mutation in A226V, D284E and A316V. Phylogenetic analysis showed that our strain shared the greatest homology with a strain isolated in Taiwan, which was derived from a strain from Indonesia. Chik-sy/2012 is in a different clade from other CHIK viruses found in China previously. A modified cDNA-AFLP in virus discovery was used to isolate the first CHIK and the first complete genome sequence of virus strain chik-sy/2012 in 2012 from a patient with CHIK fever in Zhejiang, China. The infection displayed great phylogenetic distance from viruses detected in Guangdong, China, in 2008 and 2010, since they were derived from another evolutionary lineage. Additional molecular epidemiology data are needed to further understand, monitor and evaluate CHIK in China.
登录
查看更多内容
影响因子：
5.4
作者：
Volk, Sara M.;Chen, Rubing;Weaver, Scott C.
通讯作者：
Weaver, Scott C.
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
中国广东分离的基孔肯雅病毒的完整基因组序列
影响因子：
5.4
作者：
Li, Xiao-Feng;Jiang, Tao;Qin, Cheng-Feng
通讯作者：
Qin, Cheng-Feng
影响因子：
4.8
作者：
Zheng K;Li J;Zhang Q;Liang M;Li C;Lin M;Huang J;Li H;Xiang D;Wang N;Hong Y;Huang L;Li X;Pan D;Song W;Dai J;Guo B;Li D
通讯作者：
Li D
影响因子：
3.2
作者：
Lo Presti, Alessandra;Ciccozzi, Massimo;Rezza, Giovanni
通讯作者：
Rezza, Giovanni