Organization of myelin in the mouse somatosensory barrel cortex and the effects of sensory deprivation.
Organization of myelin in the mouse somatosensory barrel cortex and the effects of sensory deprivation.
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DOI：
10.1002/dneu.22060
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发表时间：
2013-04
影响因子：
3
通讯作者：
Brumberg, Joshua C.
中科院分区：
文献类型：
--
作者：
Barrera, Kyrstle;Chu, Philip;Abramowitz, Jason;Steger, Robert;Ramos, Raddy L.;Brumberg, Joshua C.
在啮齿类动物中，桶状皮层是躯体感觉皮层中的一个专门区域，负责处理来自神秘胡须的信号。我们研究了小鼠桶状皮质髓鞘形成的正常发育，以及感觉剥夺对这种模式的影响。从出生起，每隔一天修剪一侧脸的胡须，就可以达到脱毛的效果。在对照组小鼠中，髓鞘不存在，直到出生后第14天，并没有显示出突出，直到出生后第30天，成年人的髓鞘形成水平达到了出生后第二个月结束。无偏体视学被用来估计轴突密度的interbarrel间隔区和桶壁以及桶中心。髓鞘是显着更集中在interbarrel隔膜/桶壁比在桶中心在控制和感觉剥夺条件。感觉剥夺并没有影响髓鞘形成的发生，但在桶区域的有髓轴突的显着减少，并减少髓鞘鞘每个轴突的量。少突胶质细胞核标记Olig 2的可视化显示了与使用组织化学观察到的髓磷脂相似的模式，但在感觉剥夺后Olig 2+核中没有显著变化。与显示髓鞘形成较少的解剖结果一致，局部场电位显示修剪后上升时间较慢。我们的研究结果表明，髓鞘形成的发展相对较晚，可以通过感觉经验的影响。
In rodents, the barrel cortex is a specialized area within the somatosensory cortex that processes signals from the mystacial whiskers. We investigated the normal development of myelination in the barrel cortex of mice, as well as the effects of sensory deprivation on this pattern. Deprivation was achieved by trimming the whiskers on one side of the face every other day from birth. In control mice, myelin was not present until postnatal day 14 and did not show prominence until postnatal day 30; adult levels of myelination were reached by the end of the second postnatal month. Unbiased stereology was used to estimate axon density in the interbarrel septal region and barrel walls as well as the barrel centers. Myelin was significantly more concentrated in the interbarrel septa/barrel walls than in the barrel centers in both control and sensory-deprived conditions. Sensory deprivation did not impact the onset of myelination but resulted in a significant decrease in myelinated axons in the barrel region and decreased the amount of myelin ensheathing each axon. Visualization of the oligodendrocyte nuclear marker Olig2 revealed a similar pattern of myelin as seen using histochemistry, but with no significant changes in Olig2+ nuclei following sensory deprivation. Consistent with the anatomical results showing less myelination, local field potentials revealed slower rise times following trimming. Our results suggest that myelination develops relatively late and can be influenced by sensory experience.
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影响因子：
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