Aging-associated exacerbation in fatty degeneration and infiltration after rotator cuff tear.
Aging-associated exacerbation in fatty degeneration and infiltration after rotator cuff tear.
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DOI：
10.1016/j.jse.2013.04.011
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发表时间：
2014-01
影响因子：
3
通讯作者：
Mendias, Christopher L.
中科院分区：
文献类型：
--
作者：
Gumucio, Jonathan P.;Korn, Michael A.;Saripalli, Anjali L.;Flood, Michael D.;Phan, Anthony C.;Roche, Stuart M.;Lynch, Evan B.;Claflin, Dennis R.;Bedi, Asheesh;Mendias, Christopher L.
肩袖撕裂是最常见的肌肉骨骼疾病之一，也是老年患者发病的主要原因之一。慢性袖带撕裂与肌肉萎缩和脂肪浸润有关，这种情况被称为“脂肪变性”。为了改善老年患者袖带撕裂的治疗，更好地了解肌肉纤维收缩特性的变化和脂肪变性的分子调节是必不可少的。采用老年大鼠全层、块状冈上肌和冈下肌撕裂模型，我们测量了撕裂后30天纤维收缩性和纤维类型分布的变化。我们还测量了参与肌肉萎缩、脂质积累和基质合成的mRNA和miRNA转录物的表达。我们假设在撕裂的袖带肌中会出现肌纤维比力的降低、IIb型纤维的积累以及萎缩、纤维化和炎症基因表达的上调。撕裂后30天，我们观察到肌纤维力量减少，并诱导调节萎缩、纤维化、脂质积累、炎症和巨噬细胞募集的RNA分子。观察到晚期糖基化终产物的显著积累，以及脂肪积累区域巨噬细胞的显著增加。老年大鼠退行性改变的程度大于成年大鼠。此外，我们发现慢性袖带撕裂中发生的异位脂肪积累不是通过激活典型的细胞内脂质储存和合成途径发生的。
Rotator cuff tears are one of the most common musculoskeletal complaints and a substantial source of morbidity in elderly patients. Chronic cuff tears are associated with muscle atrophy and an infiltration of fat to the area, a condition known as “fatty degeneration.” To improve the treatment of cuff tears in elderly patients, a greater understanding of the changes in the contractile properties of muscle fibers and the molecular regulation of fatty degeneration is essential. Using a full-thickness, massive supraspinatus and infraspinatus tear model in elderly rats, we measured fiber contractility and determined changes in fiber type distribution that develop 30 days after tear. We also measured the expression of mRNA and miRNA transcripts involved in muscle atrophy, lipid accumulation, and matrix synthesis. We hypothesized that a decrease in specific force of muscle fibers, an accumulation of type IIb fibers, and an upregulation in atrophic, fibrogenic, and inflammatory gene expression would occur in torn cuff muscles. Thirty days following tear, we observed a reduction in muscle fiber force and an induction of RNA molecules that regulate atrophy, fibrosis, lipid accumulation, inflammation and macrophage recruitment. A marked accumulation of advanced glycation end products, and a significant accretion of macrophages in areas of fat accumulation were observed. The extent of degenerative changes in old rats was greater than that observed in adults. Additionally, we identified that the ectopic fat accumulation that occurs in chronic cuff tears does not occur by activation of canonical intramyocellular lipid storage and synthesis pathways.
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影响因子：
5.5
作者：
Bottinelli, R;Canepari, M;Reggiani, C
通讯作者：
Reggiani, C
影响因子：
5.3
作者：
Mannava, Sandeep;Plate, Johannes F.;Tuohy, Christopher J.
通讯作者：
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DOI：
10.2106/00004623-200003000-00002
发表时间：
2000-03-01
影响因子：
5.3
作者：
Jost, B;Pfirrmann, CWA;Gerber, C
通讯作者：
Gerber, C
DOI：
10.1093/gerona/56.5.b224
发表时间：
2001-05-01
影响因子：
5.1
作者：
Carlson, BM;Dedkov, EI;Faulkner, JA
通讯作者：
Faulkner, JA
影响因子：
4.8
作者：
Cassese, Angela;Esposito, Iolanda;Miele, Claudia
通讯作者：
Miele, Claudia