Function of JARID2 in bovines during early embryonic development.
Function of JARID2 in bovines during early embryonic development.
复制标题
JARID2 在牛早期胚胎发育过程中的功能
作者：
Fu Y;Xu JJ;Sun XL;Jiang H;Han DX;Liu C;Gao Y;Yuan B;Zhang JB
组蛋白赖氨酸修饰是早期胚胎发育过程中重要的表观遗传修饰。JARID2是jumonji去甲基酶蛋白家族的成员，是早期胚胎发育的调节因子，可以通过修饰组蛋白赖氨酸来调节小鼠发育和胚胎干细胞（ESC）分化。JARID2可以通过调节H3K27me3的甲基化水平影响早期胚胎发育，这与正常的早期胚胎发育密切相关。为了研究JARID2在牛卵母细胞和早期胚胎中的表达模式以及JARID2诱导的H3K27甲基化对牛卵母细胞和早期胚胎的影响，本研究采用qRT-PCR和免疫荧光技术检测了JARID2 mRNA在牛卵母细胞和早期胚胎中的表达和定位。结果表明，JARID2在生发囊（GV）、MII、2-细胞、4-细胞、8-细胞、16-细胞和囊胚期均有高表达，但JARID2在牛GV卵母细胞中的相对表达量显著低于其他卵母细胞/胚胎期（p < 0.05），且主要在细胞核中表达。接下来，我们通过JARID2-2830- sirna显微注射检测JARID2敲除后胚胎发育相关基因（OCT4、SOX2和c-myc）的mRNA表达水平，探讨JARID2在胚胎早期发育过程中调控H3K27me3的分子途径。结果表明，这些基因在2细胞胚胎中的相对表达量显著高于囊胚期，且敲除JARID2后表达量显著增加。综上所述，本研究确定了JARID2在牛卵母细胞和早期胚胎各阶段的表达模式，结果表明JARID2通过修饰H3K27me3表达调节OCT4、SOX2和c-myc的表达，在早期胚胎发育中起关键作用。本研究为提高体外胚胎培养效率提供了新的数据，也为进一步研究早期胚胎发育的调控机制提供了理论基础。
Histone lysine modifications are important epigenetic modifications in early embryonic development. JARID2, which is a member of the jumonji demethylase protein family, is a regulator of early embryonic development and can regulate mouse development and embryonic stem cell (ESC) differentiation by modifying histone lysines. JARID2 can affect early embryonic development by regulating the methylation level of H3K27me3, which is closely related to normal early embryonic development. To investigate the expression pattern of JARID2 and the effect of JARID2-induced H3K27 methylation in bovine oocytes and early embryonic stages, JARID2 mRNA expression and localization were detected in bovine oocytes and early embryos via qRT-PCR and immunofluorescence in the present study. The results showed that JARID2 is highly expressed in the germinal vesicle (GV), MII, 2-cell, 4-cell, 8-cell, 16-cell and blastocyst stages, but the relative expression level of JARID2 in bovine GV oocytes is significantly lower than that at other oocyte/embryonic stages (p < 0.05), and JARID2 is expressed primarily in the nucleus. We next detected the mRNA expression levels of embryonic development-related genes (OCT4, SOX2 and c-myc) after JARID2 knockdown through JARID2-2830-siRNA microinjection to investigate the molecularpathwayunderlying the regulation of H3K27me3 by JARID2 during early embryonic development. The results showed that the relative expression levels of these genes in 2-cell embryos weresignificantly higher than those in the blastocyst stage, and expression levels were significantly increased after JARID2 knockdown. In summary, the present study identified the expression pattern of JARID2 in bovine oocytes and at each early embryonic stage, and the results suggest that JARID2 plays a key role in early embryonic development by regulating the expression of OCT4, SOX2 and c-myc via modification of H3K27me3 expression. This work provides new data for improvements in the efficiency of in vitro embryo culture as well as a theoretical basis for further studying the regulatory mechanisms involved in early embryonic development.
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