Genome-wide identification and comprehensive analyses of the kinomes in four pathogenic microsporidia species.
Genome-wide identification and comprehensive analyses of the kinomes in four pathogenic microsporidia species.
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四种致病性微孢子虫物种的激酶组的全基因组鉴定和综合分析。
DOI：
10.1371/journal.pone.0115890
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Li Z;Hao Y;Wang L;Xiang H;Zhou Z
微孢子虫感染的宿主范围广泛，从无脊椎动物到脊椎动物，给人类带来严重的疾病，给畜牧业带来巨大的经济损失，因此引起了人们的广泛关注。目前还没有预期的药物来对抗这种病原体。真核蛋白激酶（ePKs）在调节许多重要的细胞过程中起核心作用，因此是潜在的药物靶点。本研究对比氏肠胞虫、兔脑胞虫、家蚕微孢子虫和中华微孢子虫四种微孢子虫的蛋白激酶进行了全面的总结和比较分析。结果表明，大肠杆菌中存在34种ePKs和4种非典型蛋白激酶（aPKs）。bieneusi，29个ePKs和6个aPKs。兔的ePKs为41个，aPKs为5个; bombycis的ePKs为27个，aPKs为4个;当然。这些数据支持了微孢子虫激酶组是最小的真核细胞激酶组的结论。微孢子虫激酶组仅含有丝氨酸-苏氨酸激酶，不含受体样和酪氨酸激酶。许多与营养和能量信号以及应激反应相关的激酶在微孢子虫激酶组中丢失。然而，细胞周期，发育和生长相关的激酶，这是重要的寄生虫，是很好的保守。微孢子虫激酶组的这种减少与基因组压缩非常一致，但激酶组密度与蛋白质组大小呈负相关。此外，每个微孢子虫基因组中的蛋白激酶处于强大的纯化选择压力下。在激酶家族分类、结构域特征、增益和/或损失以及选择压力方面，这四个物种没有显著差异。虽然微孢子虫适应不同的宿主类型，但微孢子虫与宿主的协同进化并没有在蛋白激酶上得到明确的反映。总之，本研究丰富和更新了微孢子虫蛋白激酶数据库，并可能为致病性疾病的治疗设计提供有价值的信息和候选靶点。
Microsporidia have attracted considerable attention because they infect a wide range of hosts, from invertebrates to vertebrates, and cause serious human diseases and major economic losses in the livestock industry. There are no prospective drugs to counteract this pathogen. Eukaryotic protein kinases (ePKs) play a central role in regulating many essential cellular processes and are therefore potential drug targets. In this study, a comprehensive summary and comparative analysis of the protein kinases in four microsporidia–Enterocytozoon bieneusi, Encephalitozoon cuniculi, Nosema bombycis and Nosema ceranae–was performed. The results show that there are 34 ePKs and 4 atypical protein kinases (aPKs) in E. bieneusi, 29 ePKs and 6 aPKs in E. cuniculi, 41 ePKs and 5 aPKs in N. bombycis, and 27 ePKs and 4 aPKs in N. ceranae. These data support the previous conclusion that the microsporidian kinome is the smallest eukaryotic kinome. Microsporidian kinomes contain only serine-threonine kinases and do not contain receptor-like and tyrosine kinases. Many of the kinases related to nutrient and energy signaling and the stress response have been lost in microsporidian kinomes. However, cell cycle-, development- and growth-related kinases, which are important to parasites, are well conserved. This reduction of the microsporidian kinome is in good agreement with genome compaction, but kinome density is negatively correlated with proteome size. Furthermore, the protein kinases in each microsporidian genome are under strong purifying selection pressure. No remarkable differences in kinase family classification, domain features, gain and/or loss, and selective pressure were observed in these four species. Although microsporidia adapt to different host types, the coevolution of microsporidia and their hosts was not clearly reflected in the protein kinases. Overall, this study enriches and updates the microsporidian protein kinase database and may provide valuable information and candidate targets for the design of treatments for pathogenic diseases.
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影响因子：
3.5
作者：
Drewes, G;Nurse, P
通讯作者：
Nurse, P
影响因子：
4.4
作者：
Andrade LF;Nahum LA;Avelar LG;Silva LL;Zerlotini A;Ruiz JC;Oliveira G
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Oliveira G
影响因子：
30.3
作者：
Billker O;Lourido S;Sibley LD
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Sibley LD
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作者：
Angermayr, M;Roidl, A;Bandlow, W
通讯作者：
Bandlow, W
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3.6
作者：
Coccetti, P;Rossi, RL;Alberghina, L
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Alberghina, L