Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea.
Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea.
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DOI：
10.1021/cb800225v
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发表时间：
2008-12-19
影响因子：
4
通讯作者：
Viaud, Muriel
中科院分区：
文献类型：
--
作者：
Pinedo, Cristina;Wang, Chieh-Mei;Pradier, Jean-Marc;Dalmais, Berengere;Choquer, Mathias;Le Pecheur, Pascal;Morgant, Guillaume;Collado, Isidro G.;Cane, David E.;Viaud, Muriel
灰葡萄孢（Botrytis cinerea）真菌是影响200多种观赏和农业重要植物物种的经济上重要的灰霉病的病原体。B。灰霉病菌是一种坏死营养型植物病原体，其分泌非特异性植物毒素，包括倍半萜类葡萄糖苷和聚酮化合物肉毒酸。围绕先前表征的BcBOT 1基因的区域现已被鉴定为葡萄孢菌生物合成基因簇。定量RT-PCR结果显示，BcBOT 1和BcBOT 2等5个基因通过钙调神经磷酸酶信号通路共同调控。失活的BcBOT 2基因，编码一个假定的倍半萜环化酶，废除葡萄糖苷的生物合成，这可以通过反式互补恢复。BcBOT 2的失活还导致观察到具有菌株依赖性的肉毒酸的过量产生。重组BcBOT 2蛋白将法呢基二磷酸转化为葡萄孢菌生物合成途径的母体倍半萜烯，三环醇presilphiferfolan-8β-ol。
The fungus Botrytis cinerea is the causal agent of the economically important gray mold disease that affects more than 200 ornamental and agriculturally important plant species. B. cinerea is a necrotrophic plant pathogen that secretes nonspecific phytotoxins, including the sesquiterpene botrydial and the polyketide botcinic acid. The region surrounding the previously characterized BcBOT1 gene has now been identified as the botrydial biosynthetic gene cluster. Five genes including BcBOT1 and BcBOT2 were shown by quantitative Reverse Transcription-PCR to be co-regulated through the calcineurin signaling pathway. Inactivation of the BcBOT2 gene, encoding a putative sesquiterpene cyclase, abolished botrydial biosynthesis, which could be restored by in trans complementation. Inactivation of BcBOT2 also resulted in over-production of botcinic acid that was observed to be strain-dependent. Recombinant BcBOT2 protein converted farnesyl diphosphate to the parent sesquiterpene of the botrydial biosynthetic pathway, the tricyclic alcohol presilphiperfolan-8β-ol.
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影响因子：
3.6
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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