Coral microbiome database: Integration of sequences reveals high diversity and relatedness of coral-associated microbes.
Coral microbiome database: Integration of sequences reveals high diversity and relatedness of coral-associated microbes.
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DOI：
10.1111/1758-2229.12686
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发表时间：
2019-06
影响因子：
3.3
通讯作者：
Apprill A
中科院分区：
文献类型：
--
作者：
Huggett MJ;Apprill A
珊瑚相关微生物被认为在珊瑚的健康和生态中发挥着重要作用，但缺乏对特定珊瑚-微生物相互作用的了解。为了建立一个框架来检查珊瑚微生物的特异性，我们整合并遗传学比较了21，100个SSU rRNA基因桑格-从以前研究中与珊瑚相关的细菌和古细菌中产生的序列，以及伴随的宿主，位置和出版物元数据，以产生珊瑚微生物组数据库。从这个数据库中，我们确定了39个描述的和候选的细菌门和两个与珊瑚相关的细菌门，表明珊瑚是最具遗传多样性的动物微生物组之一。其次，这一新的系统发育资源表明，某些微生物确实是珊瑚特有的，包括进化上不同的宿主。具体而言，我们在细菌属内鉴定了2-37个推定的单系、珊瑚特异性序列簇，这些细菌属与最多数量的珊瑚物种（弧菌属、内共生单胞菌属和Ruegeria）以及功能相关的微生物分类群（“Amoebophilus”、“Nitrosopumilus”和未被识别的蓝藻）相关。这种系统发育资源为珊瑚及其特定微生物的更有针对性的研究提供了一个框架，这是及时的，因为需要了解珊瑚微生物组的作用及其对不断变化的海洋和珊瑚礁条件的适应性。
Coral‐associated microorganisms are thought to play a fundamental role in the health and ecology of corals, but understanding of specific coral–microbial interactions are lacking. In order to create a framework to examine coral–microbial specificity, we integrated and phylogenetically compared 21,100 SSU rRNA gene Sanger‐produced sequences from bacteria and archaea associated with corals from previous studies, and accompanying host, location and publication metadata, to produce the Coral Microbiome Database. From this database, we identified 39 described and candidate phyla of Bacteria and two Archaea phyla associated with corals, demonstrating that corals are one of the most phylogenetically diverse animal microbiomes. Secondly, this new phylogenetic resource shows that certain microorganisms are indeed specific to corals, including evolutionary distinct hosts. Specifically, we identified 2–37 putative monophyletic, coral‐specific sequence clusters within bacterial genera associated with the greatest number of coral species (Vibrio, Endozoicomonas and Ruegeria) as well as functionally relevant microbial taxa (“Candidatus Amoebophilus”, “Candidatus Nitrosopumilus” and under recognized cyanobacteria). This phylogenetic resource provides a framework for more targeted studies of corals and their specific microbial associates, which is timely given the escalated need to understand the role of the coral microbiome and its adaptability to changing ocean and reef conditions.
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影响因子：
2.4
作者：
Helmuth, BST;Timmerman, BEH;Sebens, KP
通讯作者：
Sebens, KP
影响因子：
2.5
作者：
KUHL, M;COHEN, Y;REVSBECH, NP
通讯作者：
REVSBECH, NP
影响因子：
5.1
作者：
Horn, M;Harzenetter, MD;Wagner, M
通讯作者：
Wagner, M
影响因子：
4.4
作者：
Ainsworth, TD;Fine, M;Hoegh-Guldberg, O
通讯作者：
Hoegh-Guldberg, O
影响因子：
5.1
作者：
Barott, Katie L.;Rodriguez-Brito, Beltran;Rohwer, Forest L.
通讯作者：
Rohwer, Forest L.