Uncovering of major genetic factors generating naturally occurring variation in heading date among Asian rice cultivars.
Uncovering of major genetic factors generating naturally occurring variation in heading date among Asian rice cultivars.
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DOI：
10.1007/s00122-010-1524-1
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发表时间：
2011-04
影响因子：
5.4
通讯作者：
Yano, Masahiro
中科院分区：
文献类型：
--
作者：
Ebana, Kaworu;Shibaya, Taeko;Wu, Jianzhong;Matsubara, Kazuki;Kanamori, Hiroyuki;Yamane, Hiroko;Yamanouchi, Utako;Mizubayashi, Tatsumi;Kono, Izumi;Shomura, Ayahiko;Ito, Sachie;Ando, Tsuyu;Hori, Kiyosumi;Matsumoto, Takashi;Yano, Masahiro
为了剖析控制亚洲水稻品种抽穗期自然变异的遗传因素，我们利用粳稻品种光光（Koshihikari）与来自亚洲不同地区的12个品种杂交的F2群体进行了QTL分析。这12个品种在日本筑波的自然田间条件下，抽穗期发生了变化。在10个F2组合中观察到海侵偏析。利用多杂交进行QTL分析，揭示了与花期自然变异有关的一系列位点。在每个杂交组合中检测到1 ~ 4个qtl，其中一些qtl在组合中是共享的。这些qtl的染色体位置与其他研究中检测到的染色体位置吻合良好。不同组合间qtl的等位基因效应存在差异。对Hd1、Hd3a、Hd6、RFT1和Ghd7等先前克隆的控制抽穗日期的基因进行序列分析，发现了几个功能多态性，表明这些位点的等位基因变异可能与抽穗日期的变化有关。综上所述，QTL和测序结果表明，亚洲水稻品种抽穗日期的大部分表型变异可能是由本研究中检测到的QTL的不同等位基因（可能是功能缺失和功能获得）的组合产生的。本文的在线版本（doi:10.1007/s00122-010-1524-1）包含补充材料，可供授权用户使用。
To dissect the genetic factors controlling naturally occurring variation of heading date in Asian rice cultivars, we performed QTL analyses using F2 populations derived from crosses between a japonica cultivar, Koshihikari, and each of 12 cultivars originating from various regions in Asia. These 12 diverse cultivars varied in heading date under natural field conditions in Tsukuba, Japan. Transgressive segregation was observed in 10 F2 combinations. QTL analyses using multiple crosses revealed a comprehensive series of loci involved in natural variation in flowering time. One to four QTLs were detected in each cross combination, and some QTLs were shared among combinations. The chromosomal locations of these QTLs corresponded well with those detected in other studies. The allelic effects of the QTLs varied among the cross combinations. Sequence analysis of several previously cloned genes controlling heading date, including Hd1, Hd3a, Hd6, RFT1, and Ghd7, identified several functional polymorphisms, indicating that allelic variation at these loci probably contributes to variation in heading date. Taken together, the QTL and sequencing results indicate that a large portion of the phenotypic variation in heading date in Asian rice cultivars could be generated by combinations of different alleles (possibly both loss- and gain-of-function) of the QTLs detected in this study. The online version of this article (doi:10.1007/s00122-010-1524-1) contains supplementary material, which is available to authorized users.
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