Discovery of MicroRNAs associated with myogenesis by deep sequencing of serial developmental skeletal muscles in pigs.
Discovery of MicroRNAs associated with myogenesis by deep sequencing of serial developmental skeletal muscles in pigs.
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通过对猪连续发育骨骼肌的深度测序发现与肌生成相关的 MicroRNA
DOI：
10.1371/journal.pone.0052123
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Hou X;Tang Z;Liu H;Wang N;Ju H;Li K
microRNA（miRNAs）是一类短的单链非编码RNA，通过与靶基因的3′ UTR结合来抑制靶基因的表达。这些RNA在肌生成中起关键作用。为了获得有关miRNAs参与肌发生调控的知识，从18个发育阶段收集猪最长肌（妊娠后33、40、45、50、55、60、65、70、75、80、85、90、95、100和105天胎仔，0和10天出生后的仔猪和成年猪），以使用Solexa测序技术鉴定miRNA。通过与miRBase（release 17.0）数据库中已知的miRNAs进行比较，我们检测到197个已知的miRNAs和78个新的miRNAs。此外，在110个已知的miRNAs中也观察到序列长度和单核苷酸多态性的变化。利用定量PCR（qPCR）对11种最丰富的miRNA在11种组织（背最长肌、腿部肌肉、心脏、肝脏、脾脏、肺、肾脏、胃、小肠和结肠）中的表达进行分析，结果显示ssc-miR-378、ssc-miR-1和ssc-miR-206在骨骼肌中大量表达。在骨骼肌发育过程中，ssc-miR-378的表达水平在交配后33天（dpc）较低，在65和90 dpc时增加，在出生后0天达到峰值，最终下降并保持相对稳定的水平。该表达谱表明ssc-miR-378是一种新的肌发生候选miRNA，参与了猪骨骼肌的发育。靶点预测和KEGG通路分析表明，与增殖和分化相关的骨形态发生蛋白2（BMP 2）和丝裂原活化蛋白激酶1（MAPK 1）可能是miR-378的潜在靶点。miR-378可下调猪BMP 2和MAPK 1基因3′UTR的荧光素酶活性，提示miR-378可能通过调控这两个基因来调控肌细胞的发生。
MicroRNAs (miRNAs) are short, single-stranded non-coding RNAs that repress their target genes by binding their 3′ UTRs. These RNAs play critical roles in myogenesis. To gain knowledge about miRNAs involved in the regulation of myogenesis, porcine longissimus muscles were collected from 18 developmental stages (33-, 40-, 45-, 50-, 55-, 60-, 65-, 70-, 75-, 80-, 85-, 90-, 95-, 100- and 105-day post-gestation fetuses, 0 and 10-day postnatal piglets and adult pigs) to identify miRNAs using Solexa sequencing technology. We detected 197 known miRNAs and 78 novel miRNAs according to comparison with known miRNAs in the miRBase (release 17.0) database. Moreover, variations in sequence length and single nucleotide polymorphisms were also observed in 110 known miRNAs. Expression analysis of the 11 most abundant miRNAs were conducted using quantitative PCR (qPCR) in eleven tissues (longissimus muscles, leg muscles, heart, liver, spleen, lung, kidney, stomach, small intestine and colon), and the results revealed that ssc-miR-378, ssc-miR-1 and ssc-miR-206 were abundantly expressed in skeletal muscles. During skeletal muscle development, the expression level of ssc-miR-378 was low at 33 days post-coitus (dpc), increased at 65 and 90 dpc, peaked at postnatal day 0, and finally declined and maintained a comparatively stable level. This expression profile suggested that ssc-miR-378 was a new candidate miRNA for myogenesis and participated in skeletal muscle development in pigs. Target prediction and KEGG pathway analysis suggested that bone morphogenetic protein 2 (BMP2) and mitogen-activated protein kinase 1 (MAPK1), both of which were relevant to proliferation and differentiation, might be the potential targets of miR-378. Luciferase activities of report vectors containing the 3′UTR of porcine BMP2 or MAPK1 were downregulated by miR-378, which suggested that miR-378 probably regulated myogenesis though the regulation of these two genes.
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影响因子：
3.3
作者：
MASCARELLO, F;STECCHINI, ML;BALLOCCHI, E
通讯作者：
BALLOCCHI, E
影响因子：
3.7
作者：
Cardinali B;Castellani L;Fasanaro P;Basso A;Alemà S;Martelli F;Falcone G
通讯作者：
Falcone G
影响因子：
4.8
作者：
Fujita, Yasunori;Kojima, Keitaro;Ito, Masafumi
通讯作者：
Ito, Masafumi
影响因子：
3.7
作者：
Katagiri, T;Akiyama, S;Suda, T
通讯作者：
Suda, T
DOI：
10.1016/j.bbrc.2003.12.007
发表时间：
2004-01-16
影响因子：
3.1
作者：
Lu, YH;Zi, XL;Pollak, M
通讯作者：
Pollak, M