Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania.
Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania.
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DOI：
10.3390/antibiotics10050476
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发表时间：
2021-04-21
期刊：
影响因子：
--
通讯作者：
Mshana SE
中科院分区：
文献类型：
--
作者：
Silago V;Kovacs D;Samson H;Seni J;Matthews L;Oravcová K;Lupindu AM;Hoza AS;Mshana SE
对产超广谱β-内酰胺酶（ESBL）的K. pneumoniae（ESBL-KP）和E.大肠埃希菌（ESBL-EC）携带多个ESBL基因，在我们的环境中知之甚少。本研究调查了在坦桑尼亚姆万扎的临床、定植和污染样本中同时分离的ESBL-KP和ESBL-EC中是否存在多种ESBL基因（blaCTX-M、blaTEM和blaSHV）。本研究分别成功回收了20份和55份来自先前研究的ESBL-EC和ESBL-KP样本，这些样本在−80 °C下存档。采用表型分析法对分离菌株进行ESBLs的筛选和确证，然后采用多重PCR法检测ESBLs基因。所有（100%）和97.3%的推定ESBL分离株的表型分别由临床和实验室标准化研究所（CLSI）和改良的双纸片协同方法确认。约93.3%（70/75）的ESBL菌株至少携带一种ESBL基因，其中62.9%（44/70）的ESBL菌株携带所有ESBL基因（blaCTX-M、blaTEM和blaSHV）。8对ESBL菌对抗生素的敏感性和ESBL基因的表达模式相似。从临床、定植和污染样本中同时分离出的ESBL-KP和ESBL-EC携带多种ESBL基因。此外，8对ESBL分离株的ESBL基因和抗生素敏感性模式相似，提示ESBL-KP和ESBL-EC之间存在移动的遗传元件（MGE）的传递和/或共享。
The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at −80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.
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影响因子：
14.2
作者：
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通讯作者：
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