Antibodies to synthetic peptides based on band 3 motifs react specifically with Plasmodium falciparum (humanmalaria)-infected erythrocytes and block cytoadherence
Antibodies to synthetic peptides based on band 3 motifs react specifically with Plasmodium falciparum (humanmalaria)-infected erythrocytes and block cytoadherence
复制标题
基于带 3 基序的合成肽抗体可与恶性疟原虫（人疟疾）感染的红细胞发生特异性反应并阻断细胞粘附
作者：
I. Crandall;I. Sherman
针对人阴离子转运蛋白带3的外表面环3（氨基酸546-555）和7（821-834）上的合成肽制备的兔多克隆和小鼠单克隆抗体（Mab）抑制恶性疟原虫感染的红细胞对C32无黑色素黑素瘤细胞的细胞粘附。针对外面环4（氨基酸628-642）的单克隆抗体不显著抑制粘附。鼠单克隆抗体仅识别恶性疟原虫感染的红细胞，表明环3、4和7的表位在未感染的红细胞中通常是隐蔽的。
SUMMARY Rabbit polyclonal and mouse monoclonal antibodies (Mabs) prepared against synthetic peptides patterned on exofacialloops 3 (amino acids 546–555) and 7 (821–834) of the human anion transport protein band 3 inhibited the cytoadherence of Plasmodium falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Mabs directed against exofacial loop4 (amino acids 628–642) did not inhibit adherence to a significant degree. The murine Mabs recognized only P. falciparum- infected erythrocytes suggesting that the epitopes of loops 3, 4 and 7 are normally cryptic in uninfected erythrocytes.
DOI：
10.1073/pnas.86.23.9089
发表时间：
1989-12-01
影响因子：
11.1
作者：
LUX, SE;JOHN, KM;LODISH, HF
通讯作者：
LODISH, HF
恶性疟原虫：pH 值和 Ca2 浓度对受感染红细胞与无黑色素瘤细胞的体外细胞粘附的影响。
影响因子：
2.1
作者：
Crandall,I;Smith,H;Sherman,IW
通讯作者：
Sherman,IW
DOI：
10.1073/pnas.85.15.5409
发表时间：
1988-08-01
影响因子：
11.1
作者：
TAM, JP
通讯作者：
TAM, JP