CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis.
CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis.
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CircRNA-1926通过miR-148a/b-3p/CDK19轴促进山羊SHF干细胞分化为毛囊谱系
DOI：
10.3390/ani10091552
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发表时间：
2020-09-02
期刊：
影响因子：
--
通讯作者：
Bai WL
中科院分区：
文献类型：
--
作者：
Yin RH;Zhao SJ;Jiao Q;Wang ZY;Bai M;Fan YX;Zhu YB;Bai WL
羊绒是从羊绒山羊身上提取的纤维。其纺织品以其细腻、轻盈、柔软、舒适等特点深受消费者青睐。环状rna （circRNAs）被认为在绒山羊的羊绒生长中发挥作用。CircRNA-1926先前在绒山羊中被发现，但其功能作用尚不清楚。在本研究中，我们首次证实了circRNA-1926在绒山羊的次生毛囊膨出中表达，且在毛囊生长初期表达水平显著高于休止期表达水平。接下来，我们发现circRNA-1926促进了绒山羊毛囊干细胞向毛囊谱系的分化。在机制上，我们发现circRNA-1926通过海绵miR-148a/b-3p调节CDK19的表达。我们的研究结果表明，circRNA-1926通过海绵miR-148a/b-3p促进CDK19基因的表达，促进绒山羊次生毛囊干细胞向毛囊谱系的分化。这项研究的结果为circRNA-1926在毛囊再生和羊绒生长中的功能作用提供了新的见解。环状rna （CircRNAs）是一种非编码rna，它包含一个没有5 ‘到3 ’自由末端的共价闭环结构。circrna在山羊次生毛囊（SHF）再生和羊绒生长中发挥重要作用。CircRNA-1926先前在绒山羊的SHF中被发现，但其潜在作用尚不清楚。在这项研究中，我们证实了circRNA-1926在9只绒山羊的SHF凸起中的表达，在生长前期的表达水平明显高于休止期。通过使用过表达和siRNA干扰，我们发现circRNA-1926促进羊绒山羊SHF干细胞向毛囊谱系的分化，并通过指示基因角蛋白7和角蛋白17进行了评估。通过RNA下拉，我们发现circRNA-1926与miR-148a/b-3p结合。此外，我们的数据表明circRNA-1926促进了CDK19基因的表达。通过双荧光素酶报告基因检测，发现circRNA-1926通过miR-148a/b-3p正向调节CDK19的表达。本研究结果表明，circRNA-1926通过海绵miR-148a/b-3p增强CDK19的表达，促进绒山羊SHF干细胞向毛囊谱系的分化。
Cashmere is the fiber derived from cashmere goats. Its textiles have been favored by consumers due to their typical features, like fine, light, softness, and comfort. Circular RNAs (circRNAs) are thought to play roles in cashmere growth of cashmere goats. CircRNA-1926 was previously identified in cashmere goats, but its functional roles are unclear. In this study, we firstly confirmed the expression of circRNA-1926 in secondary hair follicle bulge of cashmere goats with a significantly higher level at anagen than the counterpart of telogen. Next, we showed that circRNA-1926 promotes the differentiation of hair follicle stem cell into hair follicle lineage in cashmere goats. Mechanistically, we found that circRNA-1926 regulated the CDK19 expression via sponging miR-148a/b-3p. Our results have demonstrated that circRNA-1926 promotes the differentiation of secondary hair follicle stem cells into hair follicle lineages in cashmere goats through sponging miR-148a/b-3p to promote the expression of the CDK19 gene. The results from this study provided novel insight into the functional roles of circRNA-1926 in hair follicle regeneration and cashmere growth. Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.
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影响因子：
23.9
作者：
Folgueras AR;Guo X;Pasolli HA;Stokes N;Polak L;Zheng D;Fuchs E
通讯作者：
Fuchs E
绒山羊次级毛囊 lncRNA-HOTAIR 转录物的鉴定和分子分析揭示了整合的调控网络，其表达可能受其启动子甲基化调节
影响因子：
3.5
作者：
Jiao, Qian;Yin, Rong H.;Bai, Wen L.
通讯作者：
Bai, Wen L.
影响因子：
21.3
作者：
通讯作者：
--
DOI：
10.1261/rna.047324.114
发表时间：
2015-03
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Haerty W;Ponting CP
通讯作者：
Ponting CP
影响因子：
5.3
作者：
LAGNADO, CA;BROWN, CY;GOODALL, GJ
通讯作者：
GOODALL, GJ