Progressive genomic convergence of two Helicobacter pylori strains during mixed infection of a patient with chronic gastritis.
Progressive genomic convergence of two Helicobacter pylori strains during mixed infection of a patient with chronic gastritis.
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DOI：
10.1136/gutjnl-2014-307345
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发表时间：
2015-04
期刊：
影响因子：
24.5
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Cao Q;Didelot X;Wu Z;Li Z;He L;Li Y;Ni M;You Y;Lin X;Li Z;Gong Y;Zheng M;Zhang M;Liu J;Wang W;Bo X;Falush D;Wang S;Zhang J
目的研究幽门螺杆菌混合感染个体基因组微进化的详细性质。我们从慢性胃炎和肾炎患者的单次活检中采集了18株分离株。对这些分离株进行全基因组测序，并使用统计遗传学工具研究其进化历史。基因组分为两个分支，反映了两种不同菌株在胃中的殖民，这些谱系在估计2.8和4.2年的进化过程中积累了多样性。 我们检测到约150个明确的重组事件之间的两个分支。谱系之间的重组是一个持续进行的过程，并检测到两个分支，但在一个分支的重组效果是近一个数量级高于其他。推测的祖先序列也显示了两种菌株在多样化之前重组的证据，我们估计它们都感染了同一宿主至少12年。 谱系之间的扩增带平均长度为895 bp，并显示了在体外实验中观察到的受体序列散布的证据。 噬菌体相关蛋白的复杂的进化历史提供了证据，在过去的4年中，由一个单一的噬菌体谱系的两个分支的频繁再感染。 全基因组测序可为深入研究H.幽门螺杆菌的基础上采样细菌从单一的胃活检。
To study the detailed nature of genomic microevolution during mixed infection with multiple Helicobacter pylori strains in an individual. We sampled 18 isolates from a single biopsy from a patient with chronic gastritis and nephritis. Whole-genome sequencing was applied to these isolates, and statistical genetic tools were used to investigate their evolutionary history. The genomes fall into two clades, reflecting colonisation of the stomach by two distinct strains, and these lineages have accumulated diversity during an estimated 2.8 and 4.2 years of evolution. We detected about 150 clear recombination events between the two clades. Recombination between the lineages is a continuous ongoing process and was detected on both clades, but the effect of recombination in one clade was nearly an order of magnitude higher than in the other. Imputed ancestral sequences also showed evidence of recombination between the two strains prior to their diversification, and we estimate that they have both been infecting the same host for at least 12 years. Recombination tracts between the lineages were, on average, 895 bp in length, and showed evidence for the interspersion of recipient sequences that has been observed in in vitro experiments. The complex evolutionary history of a phage-related protein provided evidence for frequent reinfection of both clades by a single phage lineage during the past 4 years. Whole genome sequencing can be used to make detailed conclusions about the mechanisms of genetic change of H. pylori based on sampling bacteria from a single gastric biopsy.
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影响因子：
3.3
作者：
Didelot, Xavier;Falush, Daniel
通讯作者：
Falush, Daniel
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
3.7
作者：
Kulick, Stefan;Moccia, Claudia;Suerbaum, Sebastian
通讯作者：
Suerbaum, Sebastian
DOI：
10.1073/pnas.1018444108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Kennemann, Lynn;Didelot, Xavier;Suerbaum, Sebastian
通讯作者：
Suerbaum, Sebastian
影响因子：
14.9
作者：
Lagesen K;Hallin P;Rødland EA;Staerfeldt HH;Rognes T;Ussery DW
通讯作者：
Ussery DW