ferT encodes a meiosis-specific nuclear tyrosine kinase.
ferT encodes a meiosis-specific nuclear tyrosine kinase.
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ferT 编码减数分裂特异性核酪氨酸激酶。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
U. Nir
中科院分区：
文献类型：
--
作者：
B. Hazan;O. Bern;M. Carmel;F. Lejbkowicz;R. S. Goldstein;U. Nir
FERT是一种小鼠睾丸特异的mRNA，先前显示可能编码一个51kodalton的酪氨酸激酶，称为p51ferT。Fert RNA的积累仅限于处于第一次生精减数分裂前期的初级精母细胞。通过使用针对根据假定的p51ferT唯一氨基酸序列设计的合成肽的抗体，我们已经证明睾丸细胞确实含有抗p51ferT抗体识别的51kodalton蛋白质。在六只小鼠的非睾丸组织中没有检测到这种蛋白质，在14天大的小鼠的睾丸中也没有检测到它，就像Fert RNA一样。这些发现有力地表明，51kodalton蛋白是p51ferT。免疫组织化学染色显示p51ferT定位于减数分裂精母细胞。在CHO细胞中的转染实验证实了p51ferT在真核细胞中的核定位。因此，p51ferT似乎是迄今为止描述的第一个减数分裂特异的核酪氨酸激酶。
ferT is a mouse testis-specific mRNA, shown previously to potentially encode a 51 kilodalton tyrosine kinase termed p51ferT. The accumulation of ferT RNA is restricted to primary spermatocytes that are at the prophase stage of the first spermatogenic meiotic division. By using antibodies raised against a synthetic peptide which was designed according to a putative p51ferT unique amino acid sequence, we have shown that testicular cells indeed contain a 51 kilodalton protein that is recognized by the anti-p51ferT antibodies. The protein was not detected in six nontesticular mouse tissues, nor was it detected, like the ferT RNA, in the testes of 14-day-old mice. These findings strongly suggest that the 51 kilodalton protein is p51ferT. Immunohistochemical staining localized p51ferT to meiotically dividing spermatocytes. Transfection experiments in CHO cells confirmed the nuclear localization of p51ferT in eukaryotic cells. p51ferT seems thus to be the first meiosis-specific nuclear tyrosine kinase described to date.
DOI：
10.1073/pnas.82.14.4625
发表时间：
1985-01-01
影响因子：
11.1
作者：
KAMPS, MP;BUSS, JE;SEFTON, BM
通讯作者：
SEFTON, BM
通过 cDNA 文库的磷酸酪氨酸抗体筛选鉴定出新型酪氨酸激酶。
影响因子：
5.3
作者：
Kornbluth,S;Paulson,KE;Hanafusa,H
通讯作者：
Hanafusa,H