Selection for chloroquine-sensitive Plasmodium falciparum by wild Anopheles arabiensis in Southern Zambia.
Selection for chloroquine-sensitive Plasmodium falciparum by wild Anopheles arabiensis in Southern Zambia.
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DOI：
10.1186/1475-2875-12-453
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发表时间：
2013-12-19
期刊：
影响因子：
3
通讯作者：
Thuma P
中科院分区：
文献类型：
--
作者：
Mharakurwa S;Sialumano M;Liu K;Scott A;Thuma P
寄生虫抗药性的出现，特别是恶性疟原虫的出现，仍然是控制和消除疟疾的一个主要障碍。为了制定有效的公共卫生遏制策略，清楚地了解控制该领域耐药寄生虫出现和传播的因素是很重要的。目前的研究记录了赞比亚南部野生阿拉伯按蚊对氯喹敏感的疟疾寄生虫的选择。在2006年疟疾传播季节，在一个2,000平方公里的地区，使用除虫菊喷雾捕蚊器从人类卧室收集蚊子。在形态学检查和分子确认后，将媒介蚊子解剖以从腹部切片分离头部和胸部，然后进行PCR筛查恶性疟原虫感染。通过显微镜和PCR检测所有年龄段的人类居民的恶性疟原虫寄生虫病。采用PCR和限制性内切酶消化，对恶性疟原虫感染的PfCRT基因的氯喹耐药氨基酸密码子76进行基因分型。在人群中，氯喹耐药PfCRT K76 T突变体的患病率接近90%，在涂片阳性和涂片阴性（亚显微镜）感染之间的多态性无显著差异（p = 0.323，n = 128）。然而，在腹部和唾液腺阶段的感染的AN。尽管已在几周内从人类获得，但阿拉伯虫载体表现出携带野生型K76的寄生虫，具有高达9倍的更高几率（OR（95%CI）：9（3.7-20.2），p < 0.0005，n = 125）。阿拉伯按蚊在寄生虫发育的腹部和唾液腺阶段选择携带野生型K76的恶性疟原虫。快速矢量选择，最近也看到抗叶酸剂耐药性，是证据寄生虫健身成本的蚊子，并可能巩固区域异质性的出现，传播和减弱的耐药性。了解病媒选择的性质和方向有助于在综合疟疾控制和消除方案中合理遏制抗药性的传播。
The emergence of parasite drug resistance, especially Plasmodium falciparum, persists as a major obstacle for malaria control and elimination. To develop effective public health containment strategies, a clear understanding of factors that govern the emergence and spread of resistant parasites in the field is important. The current study documents selection for chloroquine-sensitive malaria parasites by wild Anopheles arabiensis in southern Zambia. In a 2,000-sq km region, mosquitoes were collected from human sleeping rooms using pyrethrum spray catches during the 2006 malaria transmission season. After morphological examination and molecular confirmation, vector mosquitoes were dissected to separate head and thorax from the abdominal section, followed by PCR screening for P. falciparum infection. Human residents of all ages were tested for P. falciparum parasitaemia by microscopy and PCR. Plasmodium falciparum infections were genotyped at the chloroquine resistance-conferring amino acid codon 76 of the PfCRT gene, using PCR and restriction enzyme digestion. In the human population there was nearly 90% prevalence of the chloroquine-resistant PfCRT K76T mutant, with no significant differences in polymorphism among smear-positive and smear-negative (submicroscopic) infections (p = 0.323, n = 128). However, infections in both abdominal and salivary gland phases of the An. arabiensis vector exhibited wild type K76-bearing parasites with up to 9X higher odds (OR (95% CI): 9 (3.7-20.2), p < 0.0005, n = 125), despite having been acquired from humans within a few weeks. Anopheles arabiensis selects for wild-type K76-bearing P. falciparum during both abdominal and salivary gland phases of parasite development. The rapid vectorial selection, also recently seen with antifolate resistance, is evidence for parasite fitness cost in the mosquito, and may underpin regional heterogeneity in the emergence, spread and waning of drug resistance. Understanding the nature and direction of vector selection could be instrumental for rational curtailment of the spread of drug resistance in integrated malaria control and elimination programmes.
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影响因子：
15.8
作者：
Carrara VI;Lwin KM;Phyo AP;Ashley E;Wiladphaingern J;Sriprawat K;Rijken M;Boel M;McGready R;Proux S;Chu C;Singhasivanon P;White N;Nosten F
通讯作者：
Nosten F
DOI：
10.1074/jbc.m111.324319
发表时间：
2012-03-23
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fisher N;Abd Majid R;Antoine T;Al-Helal M;Warman AJ;Johnson DJ;Lawrenson AS;Ranson H;O'Neill PM;Ward SA;Biagini GA
通讯作者：
Biagini GA
影响因子：
4
作者：
Kone, Aminatou;van de Vegte-Bolmer, Marga;Djimde, Abdoulaye A.
通讯作者：
Djimde, Abdoulaye A.
DOI：
10.4269/ajtmh.2012.12-0025
发表时间：
2012-08
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Fairhurst RM;Nayyar GML;Breman JG;Hallett R;Vennerstrom JL;Duong S;Ringwald P;Wellems TE;Plowe CV;Dondorp AM
通讯作者：
Dondorp AM
影响因子：
3
作者：
Frank, Matthias;Lehners, Nicola;Kremsner, Peter G.
通讯作者：
Kremsner, Peter G.