Phenotypic variability and community interactions of germinating Streptomyces spores
Phenotypic variability and community interactions of germinating Streptomyces spores
复制标题
发芽链霉菌孢子的表型变异和群落相互作用
DOI：
--
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发表时间：
2017
影响因子：
4.6
通讯作者：
Kalin H. Vetsigian
中科院分区：
文献类型：
--
作者：
Ye Xu;Kalin H. Vetsigian
在不确定的环境中，随机萌发和萌发孢子之间的相互作用可以作为有益的萌发策略。然而，关于这种现象的广泛性、物种特异性或多样性的数据很少。以链霉菌为研究对象，建立了一个量化孢子萌发和早期生长的平台。我们发现萌发过程在三个水平上是随机的：孢子的萌发时间不同，菌丝网络以不同的速度生长，一小部分孢子在萌发后不久就停止生长。此外，通过监测这些随机特性如何受到孢子密度和孢子释放的化学物质的影响，对四个物种的萌发相互作用进行了量化。随机萌发的孢子经常受到来自同一或不同物种孢子释放的化合物的促进或抑制，并且所有物种都具有不同的相互作用谱。孢子的空间分布模式很重要，孢子群的表现与单个孢子不同。老化孢子表现出较高的休眠状态，但在萌发过程中释放的化学物质存在的情况下可以有效地发芽。所有的相互作用都是特定于发芽的，只对生长速率有微弱的影响。这项工作表明，随机发芽通常受到群落环境的影响，物种适应了不同的发芽策略。
A case can be made for stochastic germination and interactions among germinating spores as beneficial germination strategies in uncertain environments. However, there is little data on how widespread, species-specific or diverse such phenomena are. Focusing on Streptomycetes, a platform was developed for quantification of germination and early growth within communities of spores. We found that the germination process is stochastic at three levels: spores vary in their germination times, mycelium networks grow at different rates, and a fraction of germlings stall their growth shortly after germination. Furthermore, by monitoring how these stochastic properties are affected by spore density and chemicals released from spores, germination interactions were quantified for four species. Stochastically germinating spores were frequently promoted or inhibited by compounds released by spores from the same or different species, and all species had distinct interaction profiles. The spatial distribution patterns were important with clusters of spores behaving differently than individual spores. Aged spores exhibited higher dormancy but could efficiently geminate in the presence of chemicals released during germination. All interactions were specific to germination and only weakly affected growth rates. This work suggests that stochastic germination is commonly affected by the community context and species have adapted diverse germination strategies.
绿色产色链霉菌孢子萌发抑制剂的特性。
DOI：
10.1099/00221287-131-4-833
发表时间：
1985
期刊：
Journal of general microbiology
影响因子：
--
作者：
Grund,AD;Ensign,JC
通讯作者：
Ensign,JC
影响因子：
15
作者：
Walsh, Christopher T.;Fischbach, Michael A.
通讯作者：
Fischbach, Michael A.