Inhibition of myoblast fusion by lysosomotropic amines.
Inhibition of myoblast fusion by lysosomotropic amines.
复制标题
溶酶胺抑制成肌细胞融合。
DOI：
10.1016/0012-1606(82)90214-7
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发表时间：
1982
影响因子：
2.7
通讯作者：
C. Kent
中科院分区：
文献类型：
--
作者：
C. Kent
几种亲溶酶体胺能抑制鸡胚成肌细胞的融合。融合的半数最大抑制所需浓度约为2 μ M（氯喹）、30 μ M（三丁胺）、3.2 mM（氯化铵）和3.3 mM（甲胺）。所有的胺抑制融合明显低于那些降低细胞密度的浓度。融合的速率和程度都受到胺的影响，胺必须在通常的融合开始前存在约20小时。通过将抑制的细胞转移到新鲜培养基中，融合的抑制是可逆的。胺不会导致不可透析的抑制剂在培养基中积累。肌酸激酶的水平增加了8倍或更多的18和65小时之间的文化与三丁胺或氯喹处理，虽然这种增加是不明显的，在控制文化。在胺处理的文化肌酸激酶活性增加主要是由于BB和MB同工酶，与MM同工酶相对较少的增加。
Fusion of cultured chick embryo myoblasts was inhibited by treatment with several lysosomotropic amines. The concentrations required for half-maximal inhibition of fusion were approximately 2 μMfor chloroquine, 30 μMfor tributylamine, 3.2 mMfor ammonium chloride, and 3.3 mMfor methylamine. All the amines inhibited fusion appreciably at concentrations lower than those that reduced cell density. Both the rate and extent of fusion were affected by the amines, which had to be present for about 20 hr before the usual onset of fusion. Inhibition of fusion was reversible by transfer of inhibited cells to fresh medium. The amines did not cause accumulation of a nondialyzable inhibitor in the culture medium. Levels of creatine kinase increased by eight-fold or more between 18 and 65 hr in cultures treated with tributylamine or chloroquine, although this increase was not as pronounced as in control cultures. The increased creatine kinase activity in amine-treated cultures was due mainly to the BB and MB isozymes, with relatively little increase in the MM isozyme.
胚胎发育过程中小鸡胸肌半乳糖基转移酶活性的变化。
DOI：
10.1042/bj1960017
发表时间：
1981
期刊：
The Biochemical journal
影响因子：
--
作者：
Ullrich,SJ;Kent,C;Carlson,DM
通讯作者：
Carlson,DM