Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge.
Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge.
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DOI：
10.1111/j.1462-2920.2008.01637.x
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发表时间：
2008-08
影响因子：
5.1
通讯作者：
Shao Z
中科院分区：
文献类型：
--
作者：
Cui Z;Lai Q;Dong C;Shao Z
深海地下环境中参与多环芳烃（PAHs）生物降解的细菌在很大程度上是未知的。为了揭示其生物多样性，在中大西洋海岭的底部表面以下2.2米，水深为3542米的沉积物采样的重力柱采样器。沉积物迅速富集原油或多环芳烃（萘，菲和芘）的混合物作为唯一的碳源，并进一步富集与上述多环芳烃混合物在实验室中。所得到的聚生体分别命名为C2CO和C2 PPN。通过平板培养、PCR-DGGE和16 S rDNA文库分析，对分离菌株的细菌组成进行了分析。在平板上，属于假交替单胞菌属、盐单胞菌属、海旋菌属和提氏菌属的菌株占主导地位。PCR-DGGE结果显示，C2CO聚生体中存在5条与Cycloclasticus、Alteromonas、Thalassospira、Alcanivorax和Rhodoclellaceae密切相关的主带，而C2 PPN聚生体中仅存在1条与Cycloclasticus相关的主带。此外，群落结构的动态响应芳香基质的变化进行了研究。结果表明，通过16 S rDNA文库分析，共检测到3种Cycloclasticus的核糖体类型，其中1种在菲降解中起关键作用; 2种Alteromonas细菌在萘重选菌群中占主导地位。虽然这两个属的细菌作为群落的主要成员生长，但它们都没有被分离出来，可能是由于它们的可培养性差。这些结果证实，细菌Cycloclasticus是重要的专性多环芳烃降解剂在海洋环境中，并与其他降解细菌，栖息在深地下沉积物的大西洋共存。这支持了这样的观点，即PAH积累和生物衰减发生在偏远地区一致和持续。
The bacteria involved in the biodegradation of polycyclic aromatic hydrocarbons (PAHs) in deep sea subsurface environments are largely unknown. In order to reveal their biodiversity, sediments from 2.2 m under the bottom surface at a water depth of 3542 m were sampled on the Middle Atlantic Ridge with a gravity column sampler. The sediments were promptly enriched with either crude oil or a mixture of PAHs (naphthalene, phenanthrene and pyrene) as the sole carbon source, and further enriched with the PAH mixture mentioned above in the lab. The resulting consortia were named C2CO and C2PPN respectively. Their bacterial composition was analysed with plate cultivation, PCR-DGGE and 16S rDNA library analysis. On plates, isolates belonging to Pseudoalteromonas, Halomonas, Marinobacter, Thalassospira and Tistrella dominated the culturable populations. With PCR-DGGE, five major bands closely related to Cycloclasticus, Alteromonas, Thalassospira, Alcanivorax and Rhodospirillaceae were detected in consortium C2CO, while only one major band of Cycloclasticus was detected in consortium C2PPN. In addition, the dynamics of community structure in response to aromatic substrate alterations were examined. As a result, three ribotypes of Cycloclasticus were detected by 16S rDNA library analysis, one which played a key role in phenanthrene degradation; two Alteromonas bacteria dominated the naphthalene reselected consortium. Although bacteria of the two genera grew as the main members of the communities, none of them were isolated, probably owing to their poor cultivability. These results confirm that bacteria of Cycloclasticus are important obligate PAH degraders in marine environments, and coexist with other degrading bacteria that inhabit the deep subsurface sediment of the Atlantic. This supports the view that PAH accumulation and bioattenuation occur in remote areas consistently and continuously.
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影响因子：
5
作者：
Gilewicz, M;Nimatuzahroh;Bertrand, JC
通讯作者：
Bertrand, JC
影响因子：
11.8
作者：
Karinen, J.F.
通讯作者：
Karinen, J.F.
影响因子：
3.4
作者：
KAWKA, OE;SIMONEIT, BRT
通讯作者：
SIMONEIT, BRT
影响因子：
4.4
作者：
Kasai, Y;Kishira, H;Harayama, S
通讯作者：
Harayama, S
影响因子：
4.4
作者：
Demanèche, S;Meyer, C;Jouanneau, Y
通讯作者：
Jouanneau, Y