Comparing the properties of electrochemical-based DNA sensors employing different redox tags.
Comparing the properties of electrochemical-based DNA sensors employing different redox tags.
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DOI：
10.1021/ac901811n
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发表时间：
2009-11-01
影响因子：
7.4
通讯作者：
White, Ryan J.
中科院分区：
文献类型：
--
作者：
Kang, Di;Zuo, Xiaolei;Yang, Renqiang;Xia, Fan;Plaxco, Kevin W.;White, Ryan J.
近年来开发的许多电化学生物传感器方法利用氧化还原标记（最常见的是亚甲蓝或二茂铁）寡核苷酸探针，该探针特异性地附着在询问电极上。据报道，此类传感器采用了一系列探针结构，包括单链和双链 DNA、更复杂的 DNA 结构、DNA 和 RNA 适体以及最近的 DNA-小分子嵌合体。这类传感器中的信号传导通常基于结合引起的效率变化，共价连接的氧化还原标记通过询问电极转移电子。在这里，我们研究了氧化还原标签的特性如何影响此类传感器的性能。具体来说，我们比较了使用二茂铁或亚甲蓝作为信号氧化还原部分制造的电化学 DNA 传感器（E-DNA 传感器）的信号传导和稳定性差异。我们发现，虽然两种标签都支持有效的 E-DNA 信号传导，但二茂铁产生的信号增益和目标亲和力略有改善。然而，这些小优势可能会付出巨大的代价：基于二茂铁的传感器远不如亚甲基蓝传感器稳定，特别是在长期储存、重复电化学询问、重复传感/再生迭代以及在复杂样品基质（如血清）中的使用方面。
Many electrochemical biosensor approaches developed in recent years utilize redox labeled (most commonly methylene blue or ferrocene) oligonucleotide probes site-specifically attached to an interrogating electrode. Sensors in this class have been reported employing a range of probe architectures, including single- and double-stranded DNA, more complex DNA structures, DNA and RNA aptamers and, most recently, DNA-small molecule chimeras. Signaling in this class of sensors is generally predicated on binding-induced changes in the efficiency with which the covalently attached redox label transfers electrons with the interrogating electrode. Here we have investigated how the properties of the redox tag affect the performance of such sensors. Specifically, we compare the differences in signaling and stability of electrochemical DNA sensors (E-DNA sensors) fabricated using either ferrocene or methylene blue as the signaling redox moiety. We find that while both tags support efficient E-DNA signaling, ferrocene produces slightly improved signal gain and target affinity. These small advantages, however, come at a potentially significant price: the ferrocene-based sensors are far less stable than their methylene blue counterparts, particularly with regards to stability to long-term storage, repeated electrochemical interrogations, repeated sensing/regeneration iterations, and employment in complex sample matrices such as blood serum.
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影响因子：
4.9
作者：
Ricci, Francesco;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
7.4
作者：
Cash, Kevin J.;Heeger, Alan J.;Plaxco, Kevin W.;Xiao, Yi
通讯作者：
Xiao, Yi
影响因子：
16.6
作者：
Xiao, Y;Lubin, AA;Plaxco, KW
通讯作者：
Plaxco, KW
影响因子：
14.8
作者：
Xiao, Yi;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1021/la800801v
发表时间：
2008-09-16
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
White RJ;Phares N;Lubin AA;Xiao Y;Plaxco KW
通讯作者：
Plaxco KW