The Establishment of a Fast and Safe Orthotopic Colon Cancer Model Using a Tissue Adhesive Technique.
The Establishment of a Fast and Safe Orthotopic Colon Cancer Model Using a Tissue Adhesive Technique.
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DOI：
10.4143/crt.2020.494
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发表时间：
2021-07
影响因子：
4.6
通讯作者：
Chang S
中科院分区：
文献类型：
--
作者：
Hu HT;Wang Z;Kim MJ;Jiang LS;Xu SJ;Jung J;Lee E;Park JH;Bakheet N;Yoon SH;Kim KY;Song HY;Chang S
我们的目的是开发一种新的原位结肠癌模型的方法，使用组织粘合剂代替传统的手术方法。用RFP HCT 116细胞建立结肠癌模型。取裸鼠皮下注射的新鲜肿瘤组织，分为A组（缝合法）和B组（组织粘附法）。A组用8-0缝合线将组织固定在近端结肠浆膜层上。B组用组织胶10 μL固定肿瘤。比较两组患者的死亡率、肿瘤种植成功率、肿瘤转移率、原发肿瘤大小及手术时间。解剖的肿瘤组织进行组织学和免疫组织化学分析。我们还进行了肿瘤标志物分析。我们观察到，使用组织粘合剂的方法，移植成功率提高30%，死亡率降低20%。平均结肠肿瘤大小显著增加了4 mm，手术时间缩短了6.5 min。H&E显示两组之间的肿瘤结构相似。癌抗原19-9、癌胚抗原、细胞角蛋白20和Ki-67的免疫组织化学染色显示两组的强度相当。实时定量逆转录分析显示，在组织粘合剂组中，9种肿瘤标志物中有8种没有变化。Western blot结果显示组织粘附剂组p-JNK（凋亡标志物）表达较低，p-MEK/p-p38（增殖标志物）表达较高。我们认为组织粘附法是一种快速、安全的原位结肠癌模型制作方法。
We aimed to develop a novel method for orthotopic colon cancer model, using tissue adhesive in place of conventional surgical method. RFP HCT 116 cell line were used to establish the colon cancer model. Fresh tumor tissue harvested from a subcutaneous injection was grafted into twenty nude mice, divided into group A (suture method) and group B (tissue adhesive method). For the group A, we fixed the tissue on the serosa layer of proximal colon by 8-0 surgical suture. For the group B, tissue adhesive (10 μL) was used to fix the tumor. The mortality, tumor implantation success, tumor metastasis, primary tumor size, and operation time were compared between the two groups. Dissected tumor tissue was analyzed for the histology and immunohistochemistry. Also, we performed tumor marker analysis. We observed 30% increase in graft success and 20% decrease in mortality, by using tissue adhesive method, respectively. The median colon tumor size was significantly increased by 4 mm and operation time was shortened by 6.5 minutes. The H&E showed similar tumor structure between the two groups. The immunohistochemistry staining for cancer antigen 19-9, carcinoembryonic antigen, cytokeratin 20, and Ki-67 showed comparable intensities in both groups. Real-time quantitative reverse transcription analysis showed eight out of nine tumor markers are unchanged in the tissue adhesive group. Western blot indicated the tissue adhesive group expressed less p-JNK (apototic marker) and more p-MEK/p-p38 (proliferation marker) levels. We concluded the tissue adhesive method is a quick and safe way to generate orthotopic, colon cancer model.
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影响因子：
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作者：
Brown KM;Xue A;Mittal A;Samra JS;Smith R;Hugh TJ
通讯作者：
Hugh TJ
影响因子：
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作者：
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254.7
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Jemal, Ahmedin
影响因子：
7.7
作者：
Girnius N;Edwards YJ;Garlick DS;Davis RJ
通讯作者：
Davis RJ