Tracking the fate of iron in early development of human blood flukes.
Tracking the fate of iron in early development of human blood flukes.
复制标题
DOI：
10.1016/j.biocel.2007.04.017
复制
发表时间：
2007
影响因子：
4
通讯作者：
Gobert, Geoffrey N.
中科院分区：
文献类型：
--
作者：
Jones, Malcolm K.;McManus, Donald P.;Sivadorai, Padma;Glanfield, Amber;Moertel, Luke;Belli, Sabina I.;Gobert, Geoffrey N.
铁（Fe）是一种重要的微量元素，存在于几乎所有的生物体中，并在许多生物反应中用作辅助因子。铁在一些无脊椎动物中的一个作用是稳定细胞外基质。人类血吸虫，日本血吸虫，是发展中国家和热带国家重大人类疾病的罪魁祸首。人类疾病是由宿主对寄生虫卵的免疫反应引起的。血吸虫在其宿主体内发育需要铁，并在雌性系统的卵黄细胞（形成蛋壳的细胞）中储存大量的铁。对铁代谢和功能的理解是其生物学的方面，可以在未来的治疗中加以利用。研究了S.研究了该元件在这些寄生虫的早期发育中的可能功能。卵黄铁储存在卵黄铁蛋白中，在雌性中上调，在卵期和成年雄性中也以低水平表达。激光显微切割显微镜，再加上逆转录酶和真实的时间PCR扩增的卵黄体铁蛋白序列，证实了卵黄细胞可能是卵黄铁蛋白的祖细胞。通过比色分析和质谱法对整个雄性和整个雌性蠕虫、卵和纯化蛋壳中的铁浓度进行评估，结果表明雌性寄生虫中的铁水平较高，但整个寄生虫卵和纯化蛋壳中的元素水平也较高。定性能量色散光谱纯化的蛋壳，揭示了铁是丰富的蛋壳，其矩阵是由高度交联的蛋壳前体蛋白。因此，卵黄商店的铁牵连在蛋壳交联扁形动物。这些观察结果强调了铁在寄生虫体代谢和卵形成中的重要性，并提出了破坏这些致病寄生虫卵形成的新途径。
Iron (Fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. One role for Fe in some invertebrates is in stabilization of extracellular matrices. The human blood fluke, Schistosoma japonicum, is responsible for significant human disease in developing and tropical nations. Disease in humans arises from host immunological reaction to parasite eggs that lodge in tissues. Schistosomes require Fe for development in their hosts, and store abundant Fe in vitelline (eggshell-forming) cells of the female system. The understanding of Fe metabolism and functionality are aspects of its biology that may be exploited in future therapeutics. The biology of Fe stores in vitelline cells of S. japonicum was investigated to illuminate possible functions of this element in early development of these parasites. Vitelline Fe is stored in yolk ferritin that is upregulated in females and is also expressed at low levels in egg-stages and adult males. Laser microdissection microscopy, coupled with reverse transcriptase- and real time-PCR amplification of schistosome ferritin sequences, confirmed that the vitelline cells are the likely progenitor cells of yolk ferritin. Assessment of Fe concentrations in whole male and whole female adult worms, eggs and purified eggshells by colorimetric assays and mass spectroscopy demonstrated higher levels of Fe in the female parasite, but also high levels of the element in whole parasite eggs and purified eggshell. Qualitative energy dispersive spectroscopy of purified eggshells, revealed that Fe is abundant in the eggshell, the matrix of which is composed of heavily cross-linked eggshell precursor proteins. Thus, vitelline stores of Fe are implicated in eggshell cross-linking in platyhelminths. These observations emphasise the importance of Fe in schistosome metabolism and egg formation and suggest new avenues for disruption of egg formation in these pathogenic parasites.
登录
查看更多内容
影响因子：
2.4
作者：
Ebersberger, I;Knobloch, J;Kunz, W
通讯作者：
Kunz, W
影响因子：
1.3
作者：
CLEMENS, LE;BASCH, PF
通讯作者：
BASCH, PF
DOI：
10.1016/0169-4758(87)90118-9
发表时间：
1987-11-01
期刊：
PARASITOLOGY TODAY
影响因子：
--
作者：
CORDINGLEY, JS
通讯作者：
CORDINGLEY, JS
影响因子：
2.4
作者：
ERASMUS, DA;POPIEL, I;SHAW, JR
通讯作者：
SHAW, JR
影响因子：
9.6
作者：
Jones, MK;Randall, LM;Engwerda, CR
通讯作者：
Engwerda, CR