Conditioned Medium from Malignant Breast Cancer Cells Induces an EMT-Like Phenotype and an Altered N-Glycan Profile in Normal Epithelial MCF10A Cells.
Conditioned Medium from Malignant Breast Cancer Cells Induces an EMT-Like Phenotype and an Altered N-Glycan Profile in Normal Epithelial MCF10A Cells.
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恶性乳腺癌细胞的条件培养基在正常上皮 MCF10A 细胞中诱导 EMT 样表型和改变的 N-聚糖谱
DOI：
10.3390/ijms18081528
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发表时间：
2017-08-01
影响因子：
5.6
通讯作者：
Guan F
中科院分区：
文献类型：
--
作者：
Guo J;Liu C;Zhou X;Xu X;Deng L;Li X;Guan F
上皮-间质转化（EMT）是肿瘤发生发展的关键过程。癌细胞和正常（非恶性）细胞之间的通信（串扰）可能促进癌症进展。从培养的癌细胞获得的条件培养基（CM）含有能够影响正常细胞的表型和行为的分泌因子。在这项研究中，正常乳腺上皮MCF 10A细胞与来自恶性乳腺癌细胞的CM（称为231-CM和453-CM）的培养物导致形态学的改变。与对照组相比，CM处理的MCF 10A细胞上皮标志物E-cadherin表达减少，间充质标志物纤连蛋白、波形蛋白、N-cadherin和TWIST 1表达增加，细胞增殖和迁移增强，细胞凋亡减少。通过MALDI-TOF/TOF-MS（基质辅助激光解吸/电离飞行时间质谱法）和凝集素微阵列分析比较231-CM处理和对照MCF 10A细胞的N-聚糖谱。经处理的细胞显示出较低水平的高甘露糖型N-聚糖结构，以及较高水平的复合型和混合型结构。通过MALDI-TOF/TOF-MS，在453-CM处理和未处理的MCF 10A细胞中也检测到改变的N-聚糖谱，并且我们发现五种岩藻糖基化的N-聚糖结构的表达在453-CM处理后的MCF 10A细胞中被抑制。（m/z 1406.663、1590.471、1668.782、2421.141和2988.342）和一个高甘露糖结构m/z 1743.722具有与231-CM处理的MCF 10A细胞相同的模式。我们的研究结果表明，来自乳腺癌细胞的CM在正常上皮细胞中诱导了EMT样过程，并改变了它们的N-聚糖谱。
Epithelial-mesenchymal transition (EMT) is a key process in cancer development and progression. Communication (crosstalk) between cancer cells and normal (nonmalignant) cells may facilitate cancer progression. Conditioned medium (CM) obtained from cultured cancer cells contains secreted factors capable of affecting phenotypes and the behaviors of normal cells. In this study, a culture of normal breast epithelial MCF10A cells with CM from malignant breast cancer cells (termed 231-CM and 453-CM) resulted in an alteration of morphology. CM-treated MCF10A, in comparison with control cells, showed a reduced expression of the epithelial marker E-cadherin, increased expression of the mesenchymal markers fibronectin, vimentin, N-cadherin, and TWIST1, meanwhile cell proliferation and migration were enhanced while cell apoptosis was decreased. N-glycan profiles of 231-CM-treated and control MCF10A cells were compared by MALDI-TOF/TOF-MS (Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry) and a lectin microarray analysis. The treated cells showed lower levels of high-mannose-type N-glycan structures, and higher levels of complex-type and hybrid-type structures. Altered N-glycan profiles were also detected in 453-CM-treated and non-treated MCF10A cells by MALDI-TOF/TOF-MS, and we found that the expression of five fucosylated N-glycan structures (m/z 1406.663, 1590.471, 1668.782, 2421.141, and 2988.342) and one high-mannose structure m/z 1743.722 have the same pattern as 231-CM-treated MCF10A cells. Our findings, taken together, show that CM derived from breast cancer cells induced an EMT-like process in normal epithelial cells and altered their N-glycan profile.
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影响因子：
3.7
作者：
Maupin KA;Sinha A;Eugster E;Miller J;Ross J;Paulino V;Keshamouni VG;Tran N;Berens M;Webb C;Haab BB
通讯作者：
Haab BB
影响因子：
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通讯作者：
Pawitan JA
影响因子：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Hakomori, Sen-itiroh
影响因子：
3.3
作者：
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通讯作者：
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