Prospect of stem cell conditioned medium in regenerative medicine.
Prospect of stem cell conditioned medium in regenerative medicine.
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DOI：
10.1155/2014/965849
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发表时间：
2014
影响因子：
--
通讯作者：
Pawitan JA
中科院分区：
文献类型：
--
作者：
Pawitan JA
背景干细胞条件培养液作为再生医学药物具有良好的前景。Objective.探讨干细胞条件培养液（CM）的制备方法，探讨其在各种疾病中的应用前景。方法.使用关键词“干细胞”和“条件培养基”或“分泌组”和“治疗”进行系统性综述。记录、分组、制表和分析关于治疗的病症/疾病、培养的细胞类型、培养细胞的培养基和补充剂、培养条件、CM处理、分析的生长因子和其他分泌物、施用方法和结果的数据。结果大多数使用CM的研究显示了良好的效果。然而，不同的CM，即使它们来自同一种细胞，也是通过不同的条件产生的，即来自不同的传代、培养基和培养条件。在一些研究中，可以获得各种类型细胞的生长因子产量，并且可以计算出生产一种应用的CM所需的细胞数量。结论各种干细胞衍生的条件培养基在各种疾病上进行了测试，大多数显示出良好的结果。然而，需要采用标准化的生产方法并对其使用进行验证。
Background. Stem cell-derived conditioned medium has a promising prospect to be produced as pharmaceuticals for regenerative medicine. Objective. To investigate various methods to obtain stem cell-derived conditioned medium (CM) to get an insight into their prospect of application in various diseases. Methods. Systematic review using keywords “stem cell” and “conditioned medium” or “secretome” and “therapy.” Data concerning treated conditions/diseases, type of cell that was cultured, medium and supplements to culture the cells, culture condition, CM processing, growth factors and other secretions that were analyzed, method of application, and outcome were noted, grouped, tabulated, and analyzed. Results. Most of CM using studies showed good results. However, the various CM, even when they were derived from the same kind of cells, were produced by different condition, that is, from different passage, culture medium, and culture condition. The growth factor yields of the various types of cells were available in some studies, and the cell number that was needed to produce CM for one application could be computed. Conclusion. Various stem cell-derived conditioned media were tested on various diseases and mostly showed good results. However, standardized methods of production and validations of their use need to be conducted.
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DOI：
10.4049/jimmunol.1003049
发表时间：
2011-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Goswami R;Kaplan MH
通讯作者：
Kaplan MH
DOI：
10.1007/s11626-013-9687-0
发表时间：
2014-02-01
影响因子：
2.1
作者：
Kim, Jumi;Lee, Ji Hye;Chae, Jung-Il
通讯作者：
Chae, Jung-Il
影响因子：
8
作者：
Mancini, A;Koch, A;Tamura, T
通讯作者：
Tamura, T
影响因子：
4.2
作者：
Cho, Yu Jin;Song, Hyeon Suk;Joo, Kyeung Min
通讯作者：
Joo, Kyeung Min
影响因子：
3.4
作者：
Chuang, Tai-Jen;Lin, Kao-Chang;Kuo, Jinn-Rung
通讯作者：
Kuo, Jinn-Rung