Msx1 Is a Regulator of Bone Formation During Development and Postnatal Growth: In Vivo Investigations in a Transgenic Mouse Model
Msx1 Is a Regulator of Bone Formation During Development and Postnatal Growth: In Vivo Investigations in a Transgenic Mouse Model
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Msx1 是发育和产后生长过程中骨形成的调节因子：转基因小鼠模型的体内研究
DOI：
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发表时间：
2002
影响因子：
2.9
通讯作者：
Ariane Berdal
中科院分区：
文献类型：
--
作者：
S. Orestes;J. Nefussi;F. Lézot;M. Oboeuf;M. Pereira;M. Mesbah;Benoît Robert;Ariane Berdal
本研究致力于利用Msx1基因敲击转基因小鼠，研究Msx1在出生至15个月龄期间的分布和功能，以及尚不清楚的事件和时期。这项研究主要集中在下颌骨，作为Msx1依赖的神经脊衍生骨骼单位的示范模型系统。该转基因系可用于研究Msx1缺失突变小鼠的形态异常和Msx1+/m杂合子小鼠的Msx1蛋白表达。在Msx1缺失突变中，最显著的特征是下颌底凸度受到抑制，牙齿和牙槽突缺失，下颌骨无软骨内成骨。出生时，在Msx1+/m杂合子动物中，我们首次发现位于下颌骨下缘的双Msx1流产-口腔和远中近端梯度场发育模式与这种骨段模型有关。Msx1的表达涉及成骨细胞和破骨细胞。骨表面有一个明显的模式：骨膜成骨细胞分化与Msx1下调有关，而在骨内膜中，分化的成骨细胞和破骨细胞都表达同源蛋白。出生后，Msx1在牙槽突和牙槽突细胞中的表达与牙齿功能密切相关。我们的数据表明，Msx1不仅在早期的模式形成中以位置特异性的方式发挥作用，而且通过作用于不同种类的骨细胞群在骨骼生长和建模中发挥作用。
The present study is devoted to Msx1 distribution and function from birth to 15 months, events and periods still unexplored in vivo using Msx1 knock in transgenic mice. The study is focused on the mandible, as an exemplary model system for Msx1-dependent neural crest-derived skeletal unit. The transgenic line enabled study of morphological abnormalities in Msx1 null mutation mice and Msx1 protein expression in Msx1+/ m heterozygous mice. In Msx1 null mutation, the most striking feature was an inhibition of the mandibular basal convexity, the absence of teeth and alveolar bone processes, and absence of endochondral ossification in the mandibular condyle. At birth, in Msx1+/ m heterozygous animals, we identified for the first time a double Msx1 aboral-oral and disto-proximal gradient field developmental pattern located in the low border of the mandibular bone in relation with this bone segment modeling. Msx1 expression involved both osteoblast and osteoclast cells. A distinct pattern characterized bone surfaces: Periosteum osteoblast differentiation was related to Msx1 downregulation, while in the endosteum both differentiated osteoblasts and osteoclasts expressed the homeoprotein. In postnatal stages, Msx1 expression was maintained in the alveolar bone processes and dento-alveolar cells in relation with tooth function. Our data suggest that Msx1 play a role in a site-specific manner not only in early patterning but also in skeletal growth and modeling by acting on heterogenous bone cell populations.
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影响因子：
4.6
作者：
YiPing Chen;Marianna Bei;I. Woo;I. Satokata;Richard L. Maas
通讯作者：
YiPing Chen;Marianna Bei;I. Woo;I. Satokata;Richard L. Maas
影响因子：
10.5
作者：
QIU, MS;BULFONE, A;RUBENSTEIN, JLR
通讯作者：
RUBENSTEIN, JLR
影响因子：
2.7
作者：
Liu, YH;Tang, ZQ;Maxson, RE
通讯作者：
Maxson, RE
Dlx5 调节鳃弓和感觉囊的区域发育。
DOI：
10.1242/dev.126.17.3831
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Depew,MJ;Liu,JK;Long,JE;Presley,R;Meneses,JJ;Pedersen,RA;Rubenstein,JL
通讯作者：
Rubenstein,JL
DOI：
10.1073/pnas.93.20.10858
发表时间：
1996-10-01
影响因子：
11.1
作者：
Stock, DW;Ellies, DL;Weiss, KM
通讯作者：
Weiss, KM