Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations.
Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations.
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DOI：
10.1186/s12864-016-3394-7
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发表时间：
2017-01-04
期刊：
影响因子：
4.4
通讯作者：
Pizzi F
中科院分区：
文献类型：
--
作者：
Capra E;Turri F;Lazzari B;Cremonesi P;Gliozzi TM;Fojadelli I;Stella A;Pizzi F
牛精液中存在的小RNA可能与精子异常和生育障碍有关。目前，常规用于精液质量评价的参数不能完全可靠地预测公牛的生育力。为了提供用于育种的冷冻保存精液的额外质量测量，开发了一种基于来自个体公牛的精子microRNA（miRNA）和Piwi相互作用RNA（皮尔纳）的深度测序的方法。为了验证我们的方法，从Percoll分离的高和低活力部分分离的精子的两个群体进行测序，其小RNA含量的特点。从4头公牛冻融精液中成功地分离出精子细胞。我们鉴定了83个miRNAs和79个推定的piRNAs簇，它们在两种组分中差异表达。40个已知差异表达的miRNAs靶向的基因通路与细胞凋亡相关。发现miR-17- 5 p、miR-26 a-5 p、miR-486- 5 p、miR-122- 5 p、miR-184和miR-20 a-5 p的失调靶向三种途径（PTEN、PI 3 K/AKT和STAT）。从单头公牛获得的小RNA测序数据与先前的发现一致。特异性miRNA在低运动精子与高运动精子中的表达存在差异，表明低运动精子中细胞功能的改变和生殖细胞凋亡的增加。本文的在线版本（doi：10.1186/s12864-016-3394-7）包含补充材料，可供授权用户使用。
Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). Small RNAs sequencing data obtained from single bulls are consistent with previous findings. Specific miRNAs are differentially represented in low versus high motile sperm, suggesting an alteration of cell functions and increased germ cell apoptosis in the low motile fraction. The online version of this article (doi:10.1186/s12864-016-3394-7) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Guan Y;Liang G;Hawken PA;Malecki IA;Cozens G;Vercoe PE;Martin GB;Guan le L
通讯作者：
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