Neurotoxic amyloid beta oligomeric assemblies recreated in microfluidic platform with interstitial level of slow flow.
Neurotoxic amyloid beta oligomeric assemblies recreated in microfluidic platform with interstitial level of slow flow.
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DOI：
10.1038/srep01921
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发表时间：
2013
影响因子：
4.6
通讯作者：
Lee, Sang-Hoon
中科院分区：
文献类型：
--
作者：
Choi, Yoon Jung;Chae, Sukyung;Kim, Jeong Hun;Barald, Kate F.;Park, Joong Yull;Lee, Sang-Hoon
阿尔茨海默病伴随着三种淀粉样蛋白的进行性、时代性变化。因此，体外模型应该为更多的生理学研究提供相同的条件。在这里，我们观察了原纤维数量随时间的变化，并研究了淀粉样蛋白部分与神经毒性之间的相关性。虽然原纤维的数量急剧增加，但神经毒性随时间的变化很小，提示原纤维对神经毒性的贡献不大。为了通过调节微环境来研究可扩散部分的神经毒性，我们创建了一个仿生微流体系统，产生可扩散低聚物的空间梯度，并评估了它们对培养神经元的影响。我们发现β淀粉样蛋白暴露会产生萎缩效应，并观察到在神经祖细胞分化过程中，连续培养的细胞会增加神经突的延伸。这些结果证明了低聚物的潜在神经毒性，并为研究β淀粉样蛋白的神经毒性建立了一个有前景的微流控平台。
Alzheimer's disease is accompanied by progressive, time-dependent changes of three moieties of amyloid beta. In vitro models therefore should provide same conditions for more physiologic studies. Here we observed changes in the number of fibrils over time and studied the correlation between amyloid beta moieties and neurotoxicity. Although the number of fibrils increased dramatically, the change in neurotoxicity with time was small, suggesting that fibrils make little contribution to neurotoxicity. To study the neurotoxicity of diffusible moieties by regulating microenvironments, we created a bio-mimetic microfluidic system generating spatial gradients of diffusible oligomeric assemblies and assessed their effects on cultured neurons. We found amyloid beta exposure produced an atrophy effect and observed neurite extension during the differentiation of neural progenitor cells increased when cells were cultured with continuous flow. The results demonstrate the potential neurotoxicity of oligomeric assemblies and establish a prospective microfluidic platform for studying the neurotoxicity of amyloid beta.
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影响因子：
64.8
作者：
Janus, C;Pearson, J;Westaway, D
通讯作者：
Westaway, D
DOI：
10.1073/pnas.90.17.7951
发表时间：
1993-09-01
影响因子：
11.1
作者：
LOO, DT;COPANI, A;COTMAN, CW
通讯作者：
COTMAN, CW
影响因子：
3.8
作者：
Stepanichev, MY;Moiseeva, YV;Gulyaeva, NV
通讯作者：
Gulyaeva, NV
影响因子：
64.8
作者：
Morgan, D;Diamond, DM;Arendash, GW
通讯作者：
Arendash, GW
影响因子：
64.8
作者：
Schenk, D;Barbour, R;Seubert, P
通讯作者：
Seubert, P