Polymer nanoparticles pass the plant interface.
Polymer nanoparticles pass the plant interface.
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DOI：
10.1038/s41467-022-35066-y
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发表时间：
2022-11-30
影响因子：
16.6
通讯作者：
Napier, Richard M.
中科院分区：
文献类型：
--
作者：
Parkinson, Sam J.;Tungsirisurp, Sireethorn;Joshi, Chitra;Richmond, Bethany L.;Gifford, Miriam L.;Sikder, Amrita;Lynch, Iseult;O'Reilly, Rachel K.;Napier, Richard M.
随着农业努力养活越来越多的人，它也必须适应越来越多的微小塑料颗粒的暴露。为了了解植物对纳米塑料的积累，我们通过水分散聚合制备了定义明确的嵌段共聚物纳米颗粒。荧光团通过腙的形成和吸收到拟南芥的根和原生质体的共聚焦显微镜进行了研究。在这里，我们表明，吸收是成反比的纳米粒子的大小。带正电荷的颗粒在根表面周围积累，并且不被根或原生质体吸收，而带负电荷的纳米颗粒缓慢积累，并且随着时间的推移在完整根的木质部中变得突出。中性纳米颗粒迅速渗透到植物根表面的完整细胞和原生质体中，但木质部负载低于阴性纳米颗粒。这些行为与动物细胞的行为不同，我们的研究结果表明，尽管有刚性细胞壁的保护，植物仍然可以接触到土壤和水中的纳米塑料。纳米塑料污染是一个严重的环境问题，可能会对植物生命产生影响，这取决于相互作用。在这里，作者研究了大小和电荷对植物根系积累和吸收模型聚合物纳米颗粒的影响，这对环境暴露和纳米颗粒向植物的输送具有影响。
As agriculture strives to feed an ever-increasing number of people, it must also adapt to increasing exposure to minute plastic particles. To learn about the accumulation of nanoplastics by plants, we prepared well-defined block copolymer nanoparticles by aqueous dispersion polymerisation. A fluorophore was incorporated via hydrazone formation and uptake into roots and protoplasts of Arabidopsis thaliana was investigated using confocal microscopy. Here we show that uptake is inversely proportional to nanoparticle size. Positively charged particles accumulate around root surfaces and are not taken up by roots or protoplasts, whereas negatively charged nanoparticles accumulate slowly and become prominent over time in the xylem of intact roots. Neutral nanoparticles penetrate rapidly into intact cells at the surfaces of plant roots and into protoplasts, but xylem loading is lower than for negative nanoparticles. These behaviours differ from those of animal cells and our results show that despite the protection of rigid cell walls, plants are accessible to nanoplastics in soil and water. Nanoplastic contamination is a serious environmental concern and could have implications on plant life depending upon interactions. Here, the authors study the effect of size and charge on the accumulation and uptake of model polymer nanoparticles by plant roots which has implications for environmental exposure and nanoparticle delivery to plants.
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DOI：
10.3390/nano11112935
发表时间：
2021-11-02
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Azeem I;Adeel M;Ahmad MA;Shakoor N;Jiangcuo GD;Azeem K;Ishfaq M;Shakoor A;Ayaz M;Xu M;Rui Y
通讯作者：
Rui Y
影响因子：
16.6
作者：
Guo P;Liu D;Subramanyam K;Wang B;Yang J;Huang J;Auguste DT;Moses MA
通讯作者：
Moses MA
影响因子：
8
作者：
Fröhlich E
通讯作者：
Fröhlich E
影响因子：
3.8
作者：
Augustine, Robin;Hasan, Anwarul;Kevadiya, Bhavesh D.
通讯作者：
Kevadiya, Bhavesh D.
影响因子：
5.6
作者：
Herrera-Ubaldo, Humberto;de Folter, Stefan
通讯作者：
de Folter, Stefan