Totipotency, pluripotency and nuclear reprogramming.
Totipotency, pluripotency and nuclear reprogramming.
复制标题
DOI：
10.1007/10_2008_45
复制
发表时间：
2009
影响因子：
--
通讯作者：
Wolf, Don
中科院分区：
文献类型：
--
作者：
Mitalipov, Shoukhrat;Wolf, Don
哺乳动物的发育始于全能受精卵，它能够发育成构成成年动物的所有特化细胞。随着发育的展开，早期胚胎的细胞增殖并分化成前两个谱系：多能内细胞团和滋养外胚层。多能细胞可以作为胚胎干细胞（ESC）以未分化状态在体外被分离、适应和无限繁殖。 ESC 保留了分化为代表三个主要胚层的细胞的能力：内胚层、中胚层或外胚层或成人体内存在的 200 多种细胞类型中的任何一种。由于许多人类疾病是由单一细胞类型的缺陷引起的，多能人类ESC代表了用于替代疗法的任何细胞或组织类型的无限来源，从而为许多破坏性病症提供了可能的治愈方法。类似于ESC的多能细胞也可以通过体细胞的核重编程通过实验衍生。重编程的体细胞可能在细胞替代疗法中发挥更重要的作用，因为患者自身的体细胞可用于重编程，从而消除移植细胞的免疫排斥。在这篇综述中，我们总结了两种主要的重编程方法：（1）体细胞核移植和（2）使用遗传操作直接重编程。
Mammalian development commences with the totipotent zygote which is capable of developing into all the specialized cells that make up the adult animal. As development unfolds, cells of the early embryo proliferate and differentiate into the first two lineages, the pluripotent inner cell mass and the trophectoderm. Pluripotent cells can be isolated, adapted and propagated indefinitely in vitro in an undifferentiated state as embryonic stem cells (ESCs). ESCs retain their ability to differentiate into cells representing the three major germ layers: endoderm, mesoderm or ectoderm or any of the 200+ cell types present in the adult body. Since many human diseases result from defects in a single cell type, pluripotent human ESCs represent an unlimited source of any cell or tissue type for replacement therapy thus providing a possible cure for many devastating conditions. Pluripotent cells resembling ESCs can also be derived experimentally by the nuclear reprogramming of somatic cells. Reprogrammed somatic cells may have an even more important role in cell replacement therapies since the patient’s own somatic cells can be used for reprogramming thereby eliminating immune based rejection of transplanted cells. In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations.
登录
查看更多内容
DOI：
10.1073/pnas.0401654101
发表时间：
2004-05-18
影响因子：
11.1
作者：
Chawengsaksophak, K;de Graaff, W;Beck, F
通讯作者：
Beck, F
影响因子：
10.5
作者：
Boiani, M;Eckardt, S;McLaughlin, KJ
通讯作者：
McLaughlin, KJ
影响因子：
2.8
作者：
EVANS, MJ;NOTARIANNI, E;MOOR, RM
通讯作者：
MOOR, RM
DOI：
10.1385/scr:1:4:293
发表时间：
2005-01-01
期刊：
STEM CELL REVIEWS
影响因子：
--
作者：
Hurlbut, William B.
通讯作者：
Hurlbut, William B.
影响因子：
2.2
作者：
JOHNSON, WH;LOSKUTOFF, NM;BETTERIDGE, KJ
通讯作者：
BETTERIDGE, KJ