Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters.
Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters.
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荷叶碱可预防仓鼠高脂肪饮食引起的肝脏脂肪变性和损伤
DOI：
10.1371/journal.pone.0063770
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun C
中科院分区：
文献类型：
--
作者：
Guo F;Yang X;Li X;Feng R;Guan C;Wang Y;Li Y;Sun C
荷叶碱是从荷叶中提取的一种主要的阿朴啡类生物碱。具有抗高脂血症、抗肥胖、抗糖尿病和促胰岛素分泌活性的荷叶。然而，目前还不清楚荷叶碱是否可以改善肝脏脂质代谢。方法/主要发现在本研究中，雄性金黄仓鼠被随机分为4组，分别饲喂正常饮食、高脂饮食（HFD）或添加荷叶碱（10和15 mg/kg·BW/d）的HFD。干预8周后，分析了仓鼠中HFD诱导的肝脏和内脏脂肪组织重量增加、血脂异常、肝脏脂肪变性和轻度坏死性炎症。荷叶碱补充保护对HFD诱导的变化，减轻坏死性炎症，并逆转代谢综合征的血清标志物喂食HFD的仓鼠。RT-PCR和蛋白质印迹分析表明，仓鼠喂食HFD有上调水平的基因相关的脂肪生成，增加游离脂肪酸浸润，并下调参与脂解和极低密度脂蛋白分泌的基因。此外，细胞色素P4502 E1和肿瘤坏死因子-α的基因表达也在HFD组中增加。荷叶碱补充剂明显抑制HFD诱导的脂质代谢相关基因表达的改变。结论/意义荷叶碱补充改善HFD诱导的血脂异常以及肝脂肪变性和损伤。荷叶碱的有益作用与参与脂质代谢的肝脏基因表达改变有关。
Background Nuciferine is a major active aporphine alkaloid from the leaves of N. nucifera Gaertn that possesses anti-hyperlipidemia, anti-hypotensive, anti-arrhythmic, and insulin secretagogue activities. However, it is currently unknown whether nuciferine can benefit hepatic lipid metabolism. Methodology/Principal Findings In the current study, male golden hamsters were randomly divided into four groups fed a normal diet, a high-fat diet (HFD), or a HFD supplemented with nuciferine (10 and 15 mg/kg·BW/day). After 8 weeks of intervention, HFD-induced increases in liver and visceral adipose tissue weight, dyslipidemia, liver steatosis, and mild necroinflammation in hamsters were analyzed. Nuciferine supplementation protected against HFD-induced changes, alleviated necroinflammation, and reversed serum markers of metabolic syndrome in hamsters fed a HFD. RT-PCR and western blot analyses revealed that hamsters fed a HFD had up-regulated levels of genes related to lipogenesis, increased free fatty acid infiltration, and down-regulated genes involved in lipolysis and very low density lipoprotein secretion. In addition, gene expression of cytochrome P4502E1 and tumor necrosis factor-α were also increased in the HFD group. Nuciferine supplementation clearly suppressed HFD-induced alterations in the expression of genes involved in lipid metabolism. Conclusions/Significance Nuciferine supplementation ameliorated HFD-induced dyslipidemia as well as liver steatosis and injury. The beneficial effects of nuciferine were associated with altered expression of hepatic genes involved in lipid metabolism.
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影响因子：
5.4
作者：
Ono, Yuka;Hattori, Eri;Ohizumi, Yasushi
通讯作者：
Ohizumi, Yasushi
影响因子：
25.7
作者：
Nozaki, Yuichi;Fujita, Koji;Nakajima, Atsushi
通讯作者：
Nakajima, Atsushi
DOI：
10.1152/ajpendo.90764.2008
发表时间：
2009-08-01
影响因子：
5.1
作者：
Basciano, Heather;Miller, Abigale E.;Adeli, Khosrow
通讯作者：
Adeli, Khosrow
影响因子：
6.1
作者：
Huang, Chun Fa;Chen, Ya Wen;Liu, Shing Hwa
通讯作者：
Liu, Shing Hwa
影响因子：
3.8
作者：
Bellentani, Stefano;Marino, Mariano
通讯作者：
Marino, Mariano