Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge.
Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge.
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DOI：
10.1021/acs.nanolett.7b03196
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发表时间：
2017-10-11
期刊：
影响因子：
10.8
通讯作者：
Edel JB
中科院分区：
文献类型：
--
作者：
Cadinu P;Paulose Nadappuram B;Lee DJ;Sze JYY;Campolo G;Zhang Y;Shevchuk A;Ladame S;Albrecht T;Korchev Y;Ivanov AP;Edel JB
越来越多的人认识到，特别是在诊断和治疗领域，单个分子中包含的信息量不仅可以更好地理解生物物理途径，而且还可以为疾病发病的早期生物标志物检测提供特殊价值。为此，已经提出了许多单分子策略，而在无标记路线方面，纳米孔传感已成为最有前途的方法之一。然而，能够在传输速率、分辨率和信噪比（SNR）方面精细地控制分子传输对于充分利用该技术的优势至关重要。在这里，我们提出了一种新的解决方案，基于一种方法，允许生物分子被单独限制在一个zeptoll纳米级液滴中，以20纳米的距离桥接两个相邻的纳米孔（纳米桥）。与传统的纳米孔相比，在纳米桥中受到限制的分子速度要慢3个数量级。这导致在信噪比，分辨率，灵敏度和检测极限显著改善。所实施的策略是通用的，正如本文所强调的，可用于检测dsDNA， RNA， ssDNA和蛋白质。
There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker detection of disease onset. To this end, numerous single molecule strategies have been proposed, and in terms of label-free routes, nanopore sensing has emerged as one of the most promising methods. However, being able to finely control molecular transport in terms of transport rate, resolution, and signal-to-noise ratio (SNR) is essential to take full advantage of the technology benefits. Here we propose a novel solution to these challenges based on a method that allows biomolecules to be individually confined into a zeptoliter nanoscale droplet bridging two adjacent nanopores (nanobridge) with a 20 nm separation. Molecules that undergo confinement in the nanobridge are slowed down by up to 3 orders of magnitude compared to conventional nanopores. This leads to a dramatic improvement in the SNR, resolution, sensitivity, and limit of detection. The strategy implemented is universal and as highlighted in this manuscript can be used for the detection of dsDNA, RNA, ssDNA, and proteins.
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