Effects of substrate stiffness on dental pulp stromal cells in culture.
Effects of substrate stiffness on dental pulp stromal cells in culture.
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DOI：
10.1002/jbm.a.36382
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Dean D
中科院分区：
文献类型：
--
作者：
Datko Williams L;Farley A;Cupelli M;Alapati S;Kennedy MS;Dean D
牙髓基质细胞（DPSC）可以分化为已知表达骨或牙本质特异性蛋白标志物的谱系。由于细胞的分化可能受到其环境的严重影响，因此有可能通过调节其生长基质的机械性能来影响DPSC的成骨/成牙潜力。在这项研究中，在21天的时间内，在包括纤连蛋白涂覆的水凝胶和玻璃基底的一系列基底上生长有和没有羟基磷灰石（HA）微粒的人DPSC，其代表约3 kPa - 50 GPa的弹性模量范围。碱性磷酸酶活性，骨桥蛋白的生产和矿化进行了监测。HA微粒的存在增加了由细胞产生的矿化基质的相对程度，相对于那些在相同的基板和介质条件下没有HA微粒。此外，培养细胞生长在较硬的基板上有较高的ALP活性和较高的矿化度比那些生长在较软的基板上。这项研究表明，DPSC的影响，其底层的生长基质的机械性能和HA微粒的存在。此外，这些培养物的显著矿化可能需要相对坚硬的基质（> 75 kPa）。
Dental pulp stromal cells (DPSCs) can be differentiated down lineages known to either express bone or dentin specific protein markers. Since the differentiation of cells can be heavily influenced by their environment, it may be possible to influence the osteogenic/odontogenic potential of DPSCs by modulating the mechanical properties of substrate on which they are grown. In this study, human DPSCs were grown with and without hydroxyapatite (HA) microparticles on a range of substrates including fibronectin-coated hydrogels and glass substrates, which represented an elastic moduli range of ~ 3 kPa – 50 GPa, over a 21 day period. Alkaline phosphatase activity, osteopontin production, and mineralization were monitored. The presence of HA microparticles increased the relative degree of mineralized matrix produced by the cells relative to those in the same substrate and media condition without the HA microparticles. In addition, cultures with cells grown on stiffer substrates had higher ALP activity and higher degree of mineralization than those grown on softer substrates. This study shows that DPSCs are affected by the mechanical properties of their underlying growth substrate and by the presence of HA microparticles. In addition, relatively stiff substrates (> 75 kPa) may be required for significant mineralization of these cultures.
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