Lidocaine suppresses mouse Peyer's Patch T cell functions and induces bacterial translocation.
Lidocaine suppresses mouse Peyer's Patch T cell functions and induces bacterial translocation.
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DOI：
10.1016/j.surg.2010.03.024
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发表时间：
2011-01
期刊：
影响因子：
3.8
通讯作者：
Chaudry, Irshad H.
中科院分区：
文献类型：
--
作者：
Kawasaki, Takashi;Kawasaki, Chika;Sata, Takeyoshi;Chaudry, Irshad H.
胃肠粘膜是微生物病原体进入的重要途径。派尔氏淋巴结 (PP) 区室的免疫细胞有助于针对感染的主动免疫反应。尽管局部麻醉药在临床实践中广泛使用，但利多卡因等局部麻醉药是否影响 PP T 细胞功能仍不清楚。本研究的目的是检查利多卡因是否对小鼠 PP T 细胞功能有影响。为了测试这一点，将新鲜分离的小鼠派尔氏淋巴集结 T 细胞与利多卡因一起孵育。评估了利多卡因对刀豆球蛋白 A 刺激的 PP T 细胞增殖和细胞因子产生的影响。还评估了利多卡因对 PP T 细胞丝裂原激活蛋白激酶 (MAPK) 激活的影响。结果表明，利多卡因抑制 PP T 细胞中的细胞增殖、细胞因子产生和 MAPK 激活。此外，我们发现体内长期暴露于利多卡因会增加 PP 中的细菌积累。利多卡因对 PP T 细胞功能增强的免疫抑制作用可能导致宿主对感染的易感性增强。
The gastrointestinal mucosa is an important route of entry for microbial pathogens. The immune cells of Peyer’s patch (PP) compartments contribute to the active immune response against infection. Although local anesthetics are widely used in clinical practice, it remains unclear whether local anesthetics such as lidocaine affect PP T cell functions. The aim of this study was to examine if lidocaine has any effects on mouse PP T cell functions. To test this, freshly isolated mouse Peyer’s patch T cells were incubated with lidocaine. The effects of lidocaine on concanavalin A-stimulated PP T cell proliferation and cytokine production were assessed. The effect of lidocaine on PP T cell mitogen-activated protein kinase (MAPK) activation was also assessed. The results indicate that lidocaine suppresses cell proliferation, cytokine production, and MAPK activation in PP T cells. Furthermore, we found that the chronic in vivo exposure to lidocaine increases bacterial accumulation in PP. The enhanced immunosuppressive effects of lidocaine on PP T cell functions could contribute to the host’s enhanced susceptibility to infection.
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DOI：
10.1016/0162-3109(85)90008-6
发表时间：
1985-01-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
--
作者：
DICKSTEIN, R;KIREMIDJIANSCHUMACHER, L;STOTZKY, G
通讯作者：
STOTZKY, G
影响因子：
3.1
作者：
Maeshirna, Yoshinori;Fukatsu, Kazuhiko;Mochizuki, Hidetaka
通讯作者：
Mochizuki, Hidetaka
影响因子：
2.1
作者：
Mikawa, K;Akamatsu, H;Niwa, Y
通讯作者：
Niwa, Y
影响因子：
6.4
作者：
Bao, S;Beagley, KW;Husband, AJ
通讯作者：
Husband, AJ
影响因子：
5.5
作者：
Maung, AA;Fujimi, S;Lederer, JA
通讯作者：
Lederer, JA