Oxidative stress and glutathione response in tissue cultures from persons with major depression.
Oxidative stress and glutathione response in tissue cultures from persons with major depression.
复制标题
DOI：
10.1016/j.jpsychires.2012.06.008
复制
发表时间：
2012-10
影响因子：
4.8
通讯作者：
Shelton, Richard C.
中科院分区：
文献类型：
--
作者：
Gibson, Sara A.;Korade, Zeljka;Shelton, Richard C.
关键词：
有证据表明，重度抑郁症（MDD）与氧化应激的外周标志物增加有关。为了探讨氧化和抗氧化反应在抑郁症，我们分析了人皮肤成纤维细胞培养物来自皮肤活检的年龄，种族和性别匹配的个人在抑郁症和正常对照组（每组n=16），培养在葡萄糖和半乳糖条件下，相对蛋白质羰基化（氧化应激的措施），谷胱甘肽还原酶（GR）的表达，和总谷胱甘肽浓度。在对照组成纤维细胞，半乳糖诱导的蛋白质羰基化和GR的葡萄糖条件下显着增加。从MDD组的细胞显示总蛋白质羰基化和GR的表达在葡萄糖条件下，显着高于对照细胞中的葡萄糖和相当于控制半乳糖。MDD细胞中从葡萄糖到半乳糖的蛋白质羰基化略有减少，GR无显著变化。任何组之间的总谷胱甘肽均无差异。增加的蛋白质羰基化和GR表达，细胞对半乳糖诱导的氧化应激的反应在对照成纤维细胞中存在于来自MDD患者的成纤维细胞中，并且不能通过抑郁患者中GR或总谷胱甘肽的减少来解释。这些研究支持氧化应激在MDD病理生理学中的作用。这些发现的进一步证实可能会导致开发新的抗氧化剂治疗抑郁症的方法。
There is evidence that major depressive disorder (MDD) is associated with increased peripheral markers of oxidative stress. To explore oxidation and antioxidant response in MDD, we assayed human dermal fibroblast cultures derived from skin biopsies of age-, race-, and sex-matched individuals in depressed and normal control groups (n=16 each group), cultured in glucose and galactose conditions, for relative protein carbonylation (a measure of oxidative stress), glutathione reductase (GR) expression, and total glutathione concentration. In control-group fibroblasts, galactose induced a significant increase from the glucose condition in both protein carbonylation and GR. The cells from the MDD group showed total protein carbonylation and GR expression in the glucose condition that was significantly higher than control cells in glucose and equivalent to controls in galactose. There was a small decrease in protein carbonylation in MDD cells from glucose to galactose and no significant change in GR. There was no difference in total glutathione among any of the groups. Increased protein carbonylation and GR expression, cellular responses to oxidative stress induced by galactose in control fibroblasts, are present in fibroblasts derived from MDD patients and are not explainable by reduced GR or total glutathione in the depressed patients. These studies support the role of oxidative stress in the pathophysiology of MDD. Further confirmation of these findings could lead to the development of novel antioxidant approaches for the treatment of depression.
登录
查看更多内容
影响因子：
3.8
作者：
Khanzode, SD;Dakhale, GN;Palasodkar, R
通讯作者：
Palasodkar, R
影响因子：
4.8
作者：
Humphries, KM;Deal, MS;Taylor, SS
通讯作者：
Taylor, SS
影响因子：
3.8
作者：
Gysin, Rene;Riederer, Irene M.;Riederer, Beat M.
通讯作者：
Riederer, Beat M.
影响因子：
6.6
作者：
Manier, DH;Shelton, RC;Sulser, F
通讯作者：
Sulser, F
DOI：
10.1073/pnas.0706778104
发表时间：
2007-10-16
影响因子：
11.1
作者：
Gysin, Rene;Kraftsik, Rudolf;Do, Kim Q.
通讯作者：
Do, Kim Q.